Waschulin Valentin, Borsetto Chiara, Corre Christophe, Wellington Elizabeth M
School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK.
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK.
Access Microbiol. 2023 Sep 12;5(9). doi: 10.1099/acmi.0.000661.v3. eCollection 2023.
γ-butyrolactone and related signalling systems are found in and other actinobacteria where they control the production of secondary or specialized metabolites such as antibiotics. Genetic manipulation of these regulatory systems therefore leads to changes in the secondary metabolite profile of a strain and has been used to activate previously silent secondary metabolite gene clusters. However, there is no easy way to assess the presence of γ-butyrolactone-like systems in strains without whole-genome sequencing. We have therefore developed and tested a PCR screen that is able to detect homologues of the commonly co-located butenolide synthase and γ-butyrolactone receptor genes. This PCR screen could be employed for the screening of strain libraries to detect signalling systems without the necessity for whole-genome sequencing.
γ-丁内酯及相关信号系统存在于链霉菌属及其他放线菌中，在这些菌中它们控制次级代谢产物或特殊代谢产物（如抗生素）的产生。因此，对这些调控系统进行基因操作会导致菌株次级代谢产物谱发生变化，并已被用于激活先前沉默的次级代谢产物基因簇。然而，在不进行全基因组测序的情况下，没有简便的方法来评估链霉菌属菌株中是否存在γ-丁内酯样系统。因此，我们开发并测试了一种聚合酶链反应（PCR）筛选方法，该方法能够检测通常共定位的丁烯内酯合酶和γ-丁内酯受体基因的同源物。这种PCR筛选方法可用于筛选菌株文库，以检测信号系统，而无需进行全基因组测序。
