通过生化和分子方法对从骆驼初乳中分离的乳酸菌潜在益生菌活性的表征
Characterization of Potential Probiotic Activity of Lactic Acid Bacteria Isolated from Camel Colostrum by Biochemical and Molecular Methods.
作者信息
Safi Enas, Haddad Moawiya, Hasan Maen, Al-Dalain Sati Y, Proestos Charalampos, Siddiqui Shahida A
机构信息
Department of Biotechnology, Faculty of Agricultural Technology, Al-Balqa Applied University, Al-Salt, Jordan.
Department of Nutrition and Food Processing, Faculty of Agricultural Technology, Al-Balqa Applied University, P.O. Box 206, Al-Salt 19117, Jordan.
出版信息
Vet Med Int. 2023 Oct 7;2023:8334152. doi: 10.1155/2023/8334152. eCollection 2023.
A total of 60 isolates of lactic acid bacteria (LAB) were isolated from Jordanian camel colostrum using biochemical and molecular methods. Two dominant species were identified, and they were and . The entire 60 isolated LAB were tested for their acidity and bile tolerance, antimicrobial activity, and antibiotic sensitivity to test their potential probiotic activity. All 60 isolates were tolerant to different pH concentrations (2, 3, 4, 5, 6, 7, 8, 9, and 10) with different survival rates (%). The entire isolates were also tolerant to different bile salt concentrations (0.2, 0.4, 0.6, 0.8, 1, 2, and 3) with different bile resistance (%). All isolates have a different range of antimicrobial activity against , , and . The 60 isolates were almost sensitive to ampicillin, amoxicillin, and clarithromycin when different concentrations were used except some isolates of intermediate resistance. Only 6% of the isolates were resistant to clarithromycin at a concentration of 15 g per disc.
使用生化和分子方法从约旦骆驼初乳中总共分离出60株乳酸菌（LAB）。鉴定出两种优势菌种，分别是 和 。对全部60株分离出的乳酸菌进行了酸度和胆汁耐受性、抗菌活性以及抗生素敏感性测试，以检验它们的潜在益生菌活性。所有60株分离菌对不同pH浓度（2、3、4、5、6、7、8、9和10）具有耐受性，且存活率不同（%）。所有分离菌对不同胆汁盐浓度（0.2、0.4、0.6、0.8、1、2和3）也具有耐受性，且胆汁抗性不同（%）。所有分离菌对 、 和 具有不同范围的抗菌活性。当使用不同浓度时，除了一些中度耐药的分离菌外，60株分离菌对氨苄西林、阿莫西林和克拉霉素几乎敏感。在每片含15 g的浓度下，只有6%的分离菌对克拉霉素耐药。
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