利用关联分析和基因分解鉴定春性面包小麦早熟性的遗传位点。
Identification of genetic loci for early maturity in spring bread wheat using the association analysis and gene dissection.
机构信息
Laboratory of Plant Molecular Genetics and Cytogenetics, The Federal State Budgetary Institution of Science Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Kurchatov Genomics Center, Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.
出版信息
PeerJ. 2023 Oct 9;11:e16109. doi: 10.7717/peerj.16109. eCollection 2023.
BACKGROUND
Early maturity in spring bread wheat is highly desirable in the regions where it enables the plants to evade high temperatures and plant pathogens at the end of the growing season.
METHODS
To reveal the genetic loci responsible for the maturity time association analysis was carried out based on phenotyping for an 11-year period and high-throughput SNP genotyping of a panel of the varieties contrasting for this trait. The expression of candidate genes was verified using qPCR. The association between the SNP markers and the trait was validated using the biparental F population.
RESULTS
Our data showed that under long-day conditions, the period from seedling to maturity is mostly influenced by the time from heading to maturity, rather than the heading time. The QTLs associated with the trait were located on 2A, 3B, 4A, 5B, 7A and 7B chromosomes with the 7BL locus being the most significant and promising for its SNPs accelerated the maturity time by about 9 days. Gene dissection in this locus detected a number of candidates, the best being (bZIP9) and (photosystem II reaction center). The two genes are predominantly expressed in the flag leaf while flowering. The effect of the SNPs was verified in F population and confirmed the association of the 4A, 5B and 7BL loci with the maturity time.
背景
在春小麦生长的地区，早期成熟是非常理想的，因为这可以使植物在生长季节结束时避开高温和病原菌。
方法
为了揭示与成熟时间相关的遗传基因座，我们进行了 11 年的表型分析，并对具有该性状差异的品种进行了高通量 SNP 基因分型。使用 qPCR 验证了候选基因的表达。使用双亲 F 群体验证了 SNP 标记与性状之间的关联。
结果
我们的数据表明，在长日照条件下，从幼苗到成熟的时间主要受从抽穗到成熟的时间影响，而不是抽穗时间。与该性状相关的 QTL 位于 2A、3B、4A、5B、7A 和 7B 染色体上，其中 7BL 位点最为显著和有前途，其 SNP 可使成熟时间提前约 9 天。在这个位点的基因剖析中检测到了一些候选基因，其中最好的是 （bZIP9）和 （photosystem II reaction center）。这两个基因在开花时主要在旗叶中表达。F 群体中的 SNP 效应得到了验证，并证实了 4A、5B 和 7BL 位点与成熟时间的关联。
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