酿酒酵母多核糖体中聚腺苷酸（Poly(A)）+ mRNA的大小

Size of poly (A) +-mRNA in Saccharomyces cerevisiae polysomes.

作者信息

Mateeva Z E, Hadjiolov A A

出版信息

Cell Biol Int Rep. 1979 Mar;3(2):129-34. doi: 10.1016/0309-1651(79)90117-6.

Abstract

The size of poly (A) +-mRNA in different classes of yeast polysomes is estimated. The average molecular weight of long-term labelled polysomal poly (A) +-mRNA is about 0,65 x 10(6) daltons. Approximately 60% of the poly (A) +-mRNA polynucleotide chains located at the 5' end, are unprotected by ribosomes and degraded by nucleases upon incubation of cell lysates, to yield a population of poly (A) +-mRNA with an average molecular weight of 0,25 x 10(6) daltons.

摘要

对不同类型酵母多核糖体中聚腺苷酸（poly(A)）+ -mRNA的大小进行了估计。长期标记的多核糖体聚腺苷酸（poly(A)）+ -mRNA的平均分子量约为0.65×10⁶道尔顿。位于5'端的大约60%的聚腺苷酸（poly(A)）+ -mRNA多核苷酸链未被核糖体保护，在细胞裂解物孵育时会被核酸酶降解，从而产生平均分子量为0.25×10⁶道尔顿的聚腺苷酸（poly(A)）+ -mRNA群体。