[来自酿酒酵母多核糖体和细胞核的聚腺苷酸RNA的大小比较（作者译）]

[Size comparison of poly (A)-RNA from polysomes and from nuclei of the yeast Saccharomyces cerevisiae (author's transl)].

作者信息

Angermann K, Grundmann U, Holz H

出版信息

Z Naturforsch C Biosci. 1976 Jan-Feb;31(1-2):85-90.

PMID:132045

Abstract

The size of poly (A)-RNA from polysomes and cell nuclei of the yeast Saccharomyces cerevisiae was investigated. Pulslabelled cells ([14C] adenin) were cracked by the French press; polysomes and nuclei were separately isolated and the RNA was finally extracted with phenol. The separation of poly (A)-containing and poly (A)-lacking fractions was achieved by oligo (dT) cellulose. These fractions were characterized by sedimentation analysis. The main portion of polysomal poly (A)-RNA sedimented with a rate of 8 to 14S, whereas the poly (A)-RNA of nuclei exhibited a sedimentation rate of 12 to 17S. Thus nuclear poly (A)-RNA is about 20-30% larger than polysomal poly (A)-RNA.

摘要

对酿酒酵母多核糖体和细胞核中聚腺苷酸（poly (A)）-RNA的大小进行了研究。用法国压榨机裂解脉冲标记的细胞（[¹⁴C]腺嘌呤）；分别分离多核糖体和细胞核，最后用苯酚提取RNA。通过寡聚（dT）纤维素实现含聚腺苷酸和不含聚腺苷酸部分的分离。通过沉降分析对这些部分进行表征。多核糖体聚腺苷酸-RNA的主要部分沉降速率为8至14S，而细胞核的聚腺苷酸-RNA沉降速率为12至17S。因此，细胞核聚腺苷酸-RNA比多核糖体聚腺苷酸-RNA大约大20 - 30%。