College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, PR China.
Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, PR China.
Anim Biotechnol. 2023 Dec;34(8):4105-4115. doi: 10.1080/10495398.2023.2266645. Epub 2024 Jan 30.
Gonadotropin-inhibitory hormone (GnIH) plays a crucial role in regulating reproduction in the hypothalamus of poultry and has been intensely investigated since its discovery. This study aimed to assess the effects of GnIH on testicular development, as well as on reproduction-related hormone release and gene expression levels in roosters. The administration of exogenous GnIH resulted in a significant reduction in testis weight, testis volume and semen quality ( < 0.05). Additionally, exogenous GnIH significantly up-regulates the expression of , and down-regulates the expression of ( < 0.05). GnIH application also decreased the , () and ()gene expression levels. Meanwhile, by neutralizing the effects of endogenous GnIH through immunization, testicular development on day 150 in roosters was significantly promoted. Compared to the control condition, GnIH immunization significantly down-regulated the expression of the and genes ( < 0.05). In conclusion, we found that exogenous GnIH treatment inhibited testicular development, reduces gene expression, and suppressed reproductive performance in roosters. Conversely, GnIH immunization down-regulated and genes, activates the reproductive system, and promotes the reproductive activity and testicular development of roosters.
促性腺激素抑制激素（GnIH）在禽类下丘脑调节生殖中发挥着重要作用，自发现以来一直受到深入研究。本研究旨在评估 GnIH 对公鸡睾丸发育以及与生殖相关的激素释放和基因表达水平的影响。外源性 GnIH 的给药导致睾丸重量、睾丸体积和精液质量显著降低（<0.05）。此外，外源性 GnIH 显著上调了 （<0.05）的表达，而下调了 （<0.05）的表达。GnIH 应用还降低了 、 （）和 （）基因的表达水平。同时，通过免疫来中和内源性 GnIH 的作用，公鸡在 150 天时的睾丸发育明显得到促进。与对照组相比，GnIH 免疫显著下调了 （<0.05）和 （<0.05）基因的表达。总之，我们发现外源性 GnIH 处理抑制了公鸡的睾丸发育，降低了 基因的表达，并抑制了公鸡的生殖性能。相反，GnIH 免疫下调了 （<0.05）和 （<0.05）基因，激活了生殖系统，促进了公鸡的生殖活动和睾丸发育。
