Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tanta University, Tanta, Egypt.
Eur Rev Med Pharmacol Sci. 2023 Oct;27(19):9401-9412. doi: 10.26355/eurrev_202310_33968.
The limitations faced by conventional drug delivery systems are being overcome through the use of rapidly evolving cancer nanotherapeutics. Determining the manner in which the Ehrlich solid tumor (EST) is impacted by the new bioactive Alanda-loaded flax seed gum nanoparticles (Alanda NPs) functioning as an anti-carcinogenic agent represents the research objective of this paper.
Identification of the functional groups, surface morphology, particle size, and zeta potential were among the characterizations and preparations made for the prepared nanoparticles. A Control group, a Flax Seed Gum group, a raw Alanda group, an Alanda NPs group, an EST group, and an induced EST treated with Alanda NPs group comprised the six groups respectively which the 60 female mice were separated into in this in vivo study.
Toxicity assessments for kidneys and liver were performed alongside the detection of total genomic DNA degradation. The zeta potential and the particle sizes for Alanda NPs were -25.60±0.38 mv and 40±0.28 nm, respectively, where the latter demonstrated a monodisperse spherical shape, per the findings. The use of Alanda NPs to treat EST was found to alle te the DNA damage, apoptosis, and renal and hepatic toxicity that EST induces. Additionally, the activation of oxidative stress and apoptosis causing the renal and hepatic toxicity induced by EST is counteracted by the scavenging of free radicals by the Alanda NPs.
A high degree of safety for effective cancer treatment was displayed by the newly developed oral nanoparticles while also demonstrating strong potential in vivo.
通过使用迅速发展的癌症纳米疗法，克服了传统药物输送系统面临的限制。本研究旨在确定新型生物活性阿拉达负载亚麻籽胶纳米粒（Alanda NPs）作为抗癌剂对艾氏腹水瘤（EST）的影响方式。
对制备的纳米粒进行了功能基团、表面形态、粒径和 Zeta 电位等表征和制备。在这项体内研究中，将 60 只雌性小鼠分别分为 6 组，分别为对照组、亚麻籽胶组、阿拉达原料药组、阿拉达 NPs 组、EST 组和用阿拉达 NPs 处理的诱导 EST 组。
对肾脏和肝脏进行了毒性评估，并检测了总基因组 DNA 降解情况。阿拉达 NPs 的 Zeta 电位和粒径分别为-25.60±0.38 mV 和 40±0.28 nm，后者呈现出单分散的球形。用阿拉达 NPs 治疗 EST 可减轻 EST 诱导的 DNA 损伤、细胞凋亡以及肾肝毒性。此外，阿拉达 NPs 通过清除自由基来拮抗 EST 诱导的肾肝毒性所引起的氧化应激和细胞凋亡的激活。
新型口服纳米粒表现出高度的安全性和有效的癌症治疗效果，同时在体内也显示出很强的潜力。
