Department of Veterinary Medicine and Animal Production, Federico II University of Naples, Naples, Italy.
Vector Borne Zoonotic Dis. 2024 Mar;24(3):135-140. doi: 10.1089/vbz.2023.0066. Epub 2023 Oct 16.
is a ubiquitous microorganism and an opportunistic pathogen responsible for numerous diseases in humans and animals, characterized by different clinical pictures with acute or subacute course. , due to its great adaptability and versatility in terms of infections and hosts, can be considered a relevant pathogen because of the harmful effects on animal health and its potential for transmission from animals to humans and vice versa. In recent years, a marked increase in multidrug-resistant has been reported, posing a serious threat for disease management, food safety, and animal and human health as they limit available therapeutic options. In light of a growing interest of the scientific community for this micro- organism and considering the limited data availability on the prevalence of this pathogen in pet rabbits, the purpose of this research was to evaluate the presence of in pet rabbits. From November 2021 to December 2022, nasal swabs were collected from 50 pet rabbits from private households in the Campania Region, southern Italy, and underwent analysis for detection. Samples were enriched in broth, then inoculated onto nutrient and selective media, including Blood agar base supplemented with 7% sheep blood and Baird-Parker Agar Base, following standard laboratory protocols. Incubations in aerobic conditions at 37°C were performed for 24/48h for colony identification. Antimicrobial susceptibility testing for all isolates was conducted using the disc diffusion method. Our results reported the presence of in 16/50 (32%) rabbits examined, showing high levels of phenotypic resistance to different antibiotics, in particular penicillin 10U (81.2%) and erythromycin 15 μg (62.5%). The study demonstrated that pet rabbits represent a significant reservoir of and contributes to the knowledge on the phenotypic antimicrobial resistance of these bacteria in rabbits raised in a domestic environment.
是一种普遍存在的微生物，也是一种机会性病原体，可导致人类和动物罹患多种疾病，其临床特征为急性或亚急性病程。由于其在感染和宿主方面具有很强的适应性和多功能性，因此可被视为一种相关病原体，因为其对动物健康造成的危害及其在动物与人类之间传播的潜力。近年来，据报道，多药耐药性 呈显著增加趋势，对疾病管理、食品安全以及动物和人类健康构成严重威胁，因为这限制了可用的治疗选择。鉴于科学界对这种微生物的兴趣日益浓厚，并且考虑到宠物兔中这种病原体的流行情况数据有限，本研究旨在评估宠物兔中 是否存在 。 2021 年 11 月至 2022 年 12 月，从意大利南部坎帕尼亚地区的 50 只私人家庭宠物兔中采集鼻拭子，并进行 检测分析。根据标准实验室方案，将样本在肉汤中富集，然后接种到营养和选择性培养基上，包括添加 7%绵羊血的血琼脂基础和 Baird-Parker 琼脂基础。在 37°C 的需氧条件下孵育 24/48 小时以进行菌落鉴定。对所有 分离株进行药敏试验，采用纸片扩散法。 我们的研究结果报告了在所检查的 50 只兔子中有 16/50（32%）携带 ，这些 对不同抗生素表现出高水平的表型耐药性，尤其是青霉素 10U（81.2%）和红霉素 15μg（62.5%）。 该研究表明，宠物兔是 的重要储存库，并为在家庭环境中饲养的兔子中这些细菌的表型抗药性提供了知识。
