Neurovascular, Metabolic, and Glymphatic Dynamics of the Brain Measured with fNIRS.

We developed a multidistance and multiwavelength continuous wave NIRS instrument to detect dynamic changes in oxygenated and deoxygenated hemoglobin (oxy- and deoxy-Hb), oxidized cytochrome-c-oxidase (oxCCO) and water of the brain and muscle. We performed measurements of the forehead during resting state and paced breathing and of the forearm during ischemic challenge in human adults. Time series analysis focusing on rhythmic signals over different time scales and different depths of the tissue revealed specific patterns of phase relationships among the signals in each of the measurement. This method can be a promising tool to understand the dynamic interaction among the neurovascular, metabolic and glymphatic system in a wide variety of subject fields.