Bohn H, Rosenstein B
J Pharmacol Methods. 1986 Nov;16(3):227-38. doi: 10.1016/0160-5402(86)90044-6.
Construction and chronic implantation of fluid-filled arterial and ventricular catheters and renal artery constrictors for testing hemodynamic drug effects in conscious dogs are described. The two catheters were made from commercially available silicone rubber and Tygon tubing connected by a molded silicone rubber plate. The blood pressure catheter was inserted via the superficial iliac artery into the abdominal aorta; and the left ventricular catheter, through the apex of the heart. Application of silicone rubber constrictors to both renal arteries resulted in the following hemodynamic values: BPs 180 +/- 4 mm Hg; BPd 111 +/- 2 mm Hg; LVPdp/dt max 3250 +/- 122 mm Hg/sec; and HR 82 +/- 4 beats/sec. Simultaneous recording of LVP and dp/dt signals with Millar Micro-Tip and fluid-filled catheters revealed a difference in signal form and size. Damping the LVP signal of the fluid-filled catheter either with a low-pass filter of 10-30 Hz or using 2-3% dextran solution as catheter fluid abolished this difference. However, a time lag and a difference in dynamic response to positive inotropic agents were still present. Based on our findings, fluid-filled ventricular catheters are recommended for routine work. The effects of antihypertensive, antianginal, and cardiotonic reference compounds could be easily detected with this methodology.
本文描述了用于检测清醒犬血流动力学药物效应的充液动脉和心室导管以及肾动脉收缩器的构建和长期植入方法。这两种导管由市售硅橡胶和通过模制硅橡胶板连接的泰贡管制成。血压导管经髂外动脉插入腹主动脉；左心室导管经心脏尖部插入。在双侧肾动脉应用硅橡胶收缩器后，得到以下血流动力学值：收缩压180±4 mmHg；舒张压111±2 mmHg；左心室压力变化率最大值3250±122 mmHg/秒；心率82±4次/秒。使用米勒微尖端导管和充液导管同时记录左心室压力（LVP）和压力变化率（dp/dt）信号，发现信号形式和大小存在差异。使用10 - 30 Hz的低通滤波器或使用2 - 3%的右旋糖酐溶液作为导管内液体来衰减充液导管的LVP信号，可消除这种差异。然而，仍存在时间延迟以及对正性肌力药物的动态反应差异。基于我们的研究结果，推荐使用充液心室导管进行常规工作。使用这种方法可以轻松检测抗高血压、抗心绞痛和强心参考化合物的作用。
