Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
BMC Res Notes. 2023 Oct 16;16(1):273. doi: 10.1186/s13104-023-06556-z.
Multiple sclerosis (MS) is a progressing neurodegenerative disease marked by chronic central nervous system inflammation and degeneration.This study investigates gene expression profiles of T-box transcription factor TBX21 (T-bet), interferon-gamma (IFN-γ), and long non-coding RNA MEG3 in peripheral blood mononuclear cells (PBMCs) from treatment-naïve Relapsing-Remitting Multiple Sclerosis patients (RRMS), healthy controls, and RRMS patients on different Disease Modifying Therapies (DMTs). The aim is to understand the role of T-bet, IFN-γ, and MEG3 in MS pathogenesis and their potential as diagnostic and therapeutic targets.
Elevated T-bet expression is observed in treatment-naïve RRMS patients compared to healthy individuals. RRMS patients treated with Interferon beta-1alpha (IFNβ-1a) and fingolimod exhibit downregulated T-bet and MEG3 expression levels, respectively, with more pronounced effects in females. Healthy individuals show a moderate positive correlation between T-bet and MEG3 and between IFN-γ and T-bet. In RRMS patients treated with Glatiramer Acetate (GA), a strong positive correlation is observed between MEG3 and IFN-γ. Remarkably, RRMS patients treated with Dimethyl Fumarate (DMF) exhibit a significant positive correlation between T-bet and MEG3. These findings underscore the diagnostic potential of T-bet in RRMS, warranting further exploration of MEG3, T-bet, and IFN-γ interplay in RRMS patients.
多发性硬化症（MS）是一种进行性神经退行性疾病，其特征为慢性中枢神经系统炎症和变性。本研究旨在探讨 T 盒转录因子 TBX21（T-bet）、干扰素-γ（IFN-γ）和长链非编码 RNA MEG3 在未经治疗的复发缓解型多发性硬化症（RRMS）患者、健康对照者和接受不同疾病修正疗法（DMT）的 RRMS 患者外周血单个核细胞（PBMCs）中的基因表达谱。目的是了解 T-bet、IFN-γ和 MEG3 在 MS 发病机制中的作用及其作为诊断和治疗靶点的潜力。
与健康个体相比，未经治疗的 RRMS 患者中 T-bet 的表达升高。接受干扰素β-1α（IFNβ-1a）和芬戈莫德治疗的 RRMS 患者分别表现出 T-bet 和 MEG3 表达水平下调，而女性的影响更为显著。健康个体中 T-bet 和 MEG3 之间以及 IFN-γ和 T-bet 之间呈中度正相关。在接受醋酸格拉替雷（GA）治疗的 RRMS 患者中，MEG3 和 IFN-γ 之间存在强烈的正相关。值得注意的是，接受富马酸二甲酯（DMF）治疗的 RRMS 患者 T-bet 和 MEG3 之间存在显著的正相关。这些发现强调了 T-bet 在 RRMS 中的诊断潜力，值得进一步探讨 MEG3、T-bet 和 IFN-γ 在 RRMS 患者中的相互作用。
