Health and medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Japan.
Int J Immunopathol Pharmacol. 2023 Jan-Dec;37:3946320231176402. doi: 10.1177/03946320231176402.
Understand the progress of inflammation over time caused by multi-walled carbon nanotubes (MWCNT).
Two types of MWCNTs were administered to C57BL/6N mice via intraperitoneal administration at low and high doses (0.05 and 1.0 mg/mouse, respectively). Inflammation was evaluated until 6 months after administration based on cytokine levels and pathological observations. The abdominal cavity lavage fluid was collected and analyzed 1 week, 1, 3, and 6 month(s) after administration. IL-6 expression markedly increased 3 months after the administration of high-dose MWCNT-7.
Notable inflammation was observed in the groups administered with one of the MWCNT, MWCNT-7. On the other hand, inflammation in another MWCNT-treated group was milder than that in the MWCNT-7-treated group. MWCNT-7 induced pronounced inflammation but did not induce tumor formation during the experimental period. Inflammation reaction is one of the most important biological responses to MWCNT.
Three months post-exposure becomes a turning point for the harmful effects of the intraperitoneally administered MWCNT-7.
了解多壁碳纳米管（MWCNT）引起的炎症随时间的进展。
将两种类型的 MWCNT 通过腹腔内给药以低剂量和高剂量（分别为 0.05 和 1.0mg/只）施用于 C57BL/6N 小鼠。基于细胞因子水平和病理观察，在给药后 6 个月内评估炎症。在给药后 1 周、1 个月、3 个月和 6 个月时收集和分析腹腔灌洗液。高剂量 MWCNT-7 给药 3 个月后，IL-6 表达明显增加。
观察到其中一种 MWCNT（MWCNT-7）给药组出现明显炎症。另一方面，另一种 MWCNT 处理组的炎症比 MWCNT-7 处理组的炎症更轻。MWCNT-7 在实验期间引起明显的炎症，但未诱导肿瘤形成。炎症反应是对 MWCNT 的最重要的生物学反应之一。
MWCNT-7 腹腔内给药 3 个月后成为其有害作用的转折点。
