Int J Oral Maxillofac Implants. 2023 Oct 17;38(5):923-932. doi: 10.11607/jomi.9822.
To compare bone substitutes composed of glycated collagen with synthetic micro-sized (1 to 10 μm) hydroxyapatite (OB) vs non-cross-linked collagen matrix with large-particle (250 to 1,000 μm) bovine-derived hydroxyapatite (BOC).
The P1 to P4 premolars were bilaterally extracted from the mandibles of 19 Beagle dogs. After 21 days, osteotomies were created in each dog that received OB or BOC and were covered with a collagen membrane or were left untreated. The animals were randomly divided into three groups based on sacrifice time (4, 12, or 24 weeks). The right and left hemimandibles were trimmed to facilitate imaging and histology, and all tissues were placed in 10% neutral-buffered formalin. Microcomputed tomography (MicroCT 40 Scanner, Scanco) was used to analyze bone sections. Bone volume, residual material volume, and bone mineral density were determined for each treatment site (OB and BOC) based on a volume of interest that encompassed the original defect. Additionally, blinded histopathologic assessment (based on the ISO 10993-6 scoring system) and histomorphometry were performed on sections ground to < 100 μm thick and stained with Stevenel's blue.
No clinical side effects were noted. No statistical differences were observed for OB vs BOC regarding the mineral volume percentage. Compared to OB, BOC had significantly higher mean mineralization densities at 12 weeks (P < .01), but this difference did not extend to 24 weeks. For residual grafting material, bone maturation, alveolar ridge restoration, and inflammatory response, OB showed a residual amount of bone graft and no statistical differences compared to BOC.
Both OB and BOC represent valid treatment options for critically sized bone defects. Both bone fillers outperformed the sham-operated, ungrafted (empty) control, demonstrating statistically improved bone growth and ridge restoration.
比较由糖化胶原组成的骨替代品与合成的微尺寸（1 至 10μm）羟基磷灰石（OB）与非交联胶原基质与大颗粒（250 至 1000μm）牛源羟基磷灰石（BOC）。
从 19 只比格犬的下颌骨中双侧取出 P1 至 P4 前磨牙。21 天后，在每只狗身上进行截骨术，接受 OB 或 BOC，并覆盖胶原膜或不做处理。根据处死时间（4、12 或 24 周），将动物随机分为三组。将右侧和左侧下颌骨修剪，以便于成像和组织学检查，并将所有组织置于 10％中性缓冲福尔马林中。使用 MicroCT 40 扫描仪（Scanco）分析骨切片。根据包含原始缺损的感兴趣体积，确定每个治疗部位（OB 和 BOC）的骨体积、残留材料体积和骨矿物质密度。此外，对研磨至 <100μm 厚并用 Stevenel 蓝染色的切片进行盲法组织病理学评估（基于 ISO 10993-6 评分系统）和组织形态计量学。
未观察到临床副作用。OB 与 BOC 相比，矿物质体积百分比无统计学差异。与 OB 相比，BOC 在 12 周时具有显著更高的平均矿化密度（P<.01），但这种差异并未延伸至 24 周。对于残留移植物、骨成熟、牙槽嵴修复和炎症反应，OB 显示出残留的移植物量，与 BOC 相比无统计学差异。
OB 和 BOC 均为临界尺寸骨缺损的有效治疗选择。两种骨填充物的表现均优于假手术、未移植（空）对照，表现出统计学上改善的骨生长和嵴修复。
