Chikwem J O, Downey R J
Microbios. 1986;47(192-193):159-63.
Four detergents (octyl glucoside, zwittergent, Triton X-100, and Nonidet P-40) were examined with regard to their efficiency in solubilizing and retaining the activity of the nitrate reductase of Bacillus stearothermophilus. At a concentration of between 0.4 and 0.6%, the non-ionic detergent octyl glucoside solubilized only 64% of the membrane proteins. However, about 100% of the nitrate reductase activity was recovered in the soluble fraction. In contrast, the zwitterionic detergent 3-(alkyldimethylammonio)-1-propanesulphonate (3-16) solubilized 100% of the membrane proteins, but only 77% of the nitrate reductase activity was recovered. Two other non-ionic detergents, Triton X-100 and Nonidet P-40 also solubilized 100% of the membrane proteins, but there was a dramatic increase in total enzyme activity following solubilization. The enzyme activity was not stable in any of the four detergents for more than 2 days. Nevertheless, octyl glucoside was preferred because of the additional advantage of ammonium sulphate fractionation.
研究了四种去污剂（辛基葡糖苷、两性离子去污剂、Triton X-100和Nonidet P-40）对嗜热脂肪芽孢杆菌硝酸还原酶的溶解效率和活性保留情况。在浓度为0.4%至0.6%时，非离子去污剂辛基葡糖苷仅溶解了64%的膜蛋白。然而，约100%的硝酸还原酶活性在可溶性部分中得以恢复。相比之下，两性离子去污剂3-(烷基二甲基铵基)-1-丙烷磺酸盐（3-16）溶解了100%的膜蛋白，但仅恢复了77%的硝酸还原酶活性。另外两种非离子去污剂Triton X-100和Nonidet P-40也溶解了100%的膜蛋白，但溶解后总酶活性急剧增加。在这四种去污剂中，酶活性在超过2天的时间内都不稳定。尽管如此，由于硫酸铵分级分离的额外优势，辛基葡糖苷仍是首选。
