Urbina J P, Marín C, Rodrigo D, González-Tejedor G
Instituto de Agroquímica y Tecnología de Alimentos, Departamento de Tecnologías de Conservación y Seguridad Alimentaria, Paterna, Valencia.
Sistema Nacional de Investigación (SNI), SENACYT, Ciudad de Panamá, Panamá.
Food Sci Technol Int. 2025 Jun;31(4):331-337. doi: 10.1177/10820132231208085. Epub 2023 Oct 17.
Edible insects currently represent an interesting alternative protein source to the animal ones. The objective of the present wok is to characterize proteins isolated from common cricket (). Powder samples of this insect-based flour were obtained using two extraction methods, i.e. acid and alkaline. Subsequently, the proteins isolated have been characterized. The fractionation of proteins in the flour of by acid or alkaline-based methods, gave rise to isolates with up to 71.6% in protein content. Extraction in an alkaline medium of insoluble proteins (pellet) resulted in the best performance on protein recovery. These isolates present a wide variety of peptides and proteins, having identified the following ones in the pellet fraction obtained with the acid method: myosin heavy-chain isoforms C, E and Miosin heavy chain (Mhc); tropomyosin; troponin; α and β actin, and some enzymes such as the β subunit ATP synthetase. The characterization results provide information which will enable us to predict the possible physicochemical (gel formation, solubility, water retention capacity, etc.) changes that could take place in the cricket protein during processing in the food and feed industry.
可食用昆虫目前是一种有趣的替代动物蛋白的蛋白质来源。本研究的目的是对从普通蟋蟀（）中分离出的蛋白质进行表征。使用两种提取方法，即酸法和碱法，获得了这种昆虫基面粉的粉末样品。随后，对分离出的蛋白质进行了表征。通过基于酸或碱的方法对蟋蟀面粉中的蛋白质进行分级分离，得到了蛋白质含量高达71.6%的分离物。在碱性介质中提取不溶性蛋白质（沉淀）在蛋白质回收率方面表现最佳。这些分离物呈现出多种肽和蛋白质，在用酸法获得的沉淀级分中鉴定出了以下几种：肌球蛋白重链异构体C、E和肌球蛋白重链（Mhc）；原肌球蛋白；肌钙蛋白；α和β肌动蛋白，以及一些酶，如β亚基ATP合成酶。表征结果提供了信息，使我们能够预测在食品和饲料工业加工过程中蟋蟀蛋白质可能发生的物理化学变化（凝胶形成、溶解性、保水能力等）。
