Department of Chemical & Biomolecular Engineering, National University of Singapore, Singapore 117585, Singapore.
Chem Commun (Camb). 2023 Nov 2;59(88):13167-13170. doi: 10.1039/d3cc02427j.
We report the impact of fluorophore-quencher labelling on the thermodynamics of hairpin opening by testing five fluorophores and two quenchers labelled at the end and/or internal positions. Two counter strategies were introduced, label the hairpin probe at an internal position or append an external hairpin stem on the trigger strand to promote coaxial stacking hybridization. The observations remained valid for complex hairpin opening operations such as hybridization chain reaction.
我们通过测试末端和/或内部位置标记的五个荧光团和两个猝灭剂，报告了荧光团-猝灭剂标记对发夹打开热力学的影响。引入了两种对策，即标记发夹探针的内部位置或在触发链上附加一个外部发夹茎，以促进同轴堆叠杂交。这些观察结果对于复杂的发夹打开操作（如杂交链式反应）仍然有效。
