St. Norbert College, De Pere, WI 54115, USA.
Biotechniques. 2023 Dec;75(6):231-239. doi: 10.2144/btn-2023-0074. Epub 2023 Oct 18.
Whole-mount hybridization is a critical technique for analyzing gene expression in planarians. While robust protocols have been developed, these protocols are laborious, making them challenging to incorporate in an academic setting, reducing throughput and increasing time to results. Here, the authors systematically tested modifications to all phases of the protocol with the goal of eliminating steps and reducing time without impacting quality. This modified protocol allows for whole-mount colorimetric hybridization and multicolor fluorescence hybridization to be completed in two days with a significant reduction in steps and hands-on processing time.
整体杂交是分析涡虫基因表达的关键技术。虽然已经开发出了强大的方案，但这些方案繁琐，难以在学术环境中采用，从而降低了通量并延长了结果时间。在这里，作者系统地测试了方案所有阶段的修改，旨在在不影响质量的情况下消除步骤并减少时间。该改良方案允许在两天内完成全组织比色杂交和多色荧光杂交，并且显著减少了步骤和手工处理时间。
