Universidade Federal de Cariri - UFCA, Centro de Ciências Agrárias e da Biodiversidade - CCAB, Crato, CE, Brasil.
Universidade Federal de Cariri - UFCA, Faculdade de Medicina - FAMED, Barbalha, CE, Brasil.
Braz J Biol. 2023 Oct 13;83:e274635. doi: 10.1590/1519-6984.274635. eCollection 2023.
This study evaluated the insecticidal activity of crude extracts from Enterolobium contortisiliquum (Vell.) seeds on eggs and larvae of A. aegypti, and also verified the phytochemical profile and the presence of lectins in the extract. The 0.15 M NaCl saline solution was used as the extracting substance. For tests with eggs and larvae, the crude extract was used in its raw form (RCE) and boiled at 100º C for 5 min (BCE). Concentrations of 4.68; 9.37; 18.75; 28.13; 37.13 and 46.89 mg/mL, with distilled water as a negative control. Assays were performed in triplicate. The results were subjected to analysis of variance, Tukey's test and Log-Probit analysis to determine LC50 and LC90. BCE showed better results on eggs than RCE, managing to prevent the hatching of larvae in 81.66% ± 10.40 of treated eggs, at a concentration of 46.89 mg/mL. The LC50 and LC90 were set at 35.95 and 52.67 mg/mL, respectively. In tests with larvae, concentrations of 46.89 and 37.13 mg/mL, for RCE and BCE, caused 100% mortality in 24 hours of exposure. Larval mortality at the other concentrations increased with exposure time extending to 48 h. RCE, at 48 h exposure is the most promising extract on larvae (E = 72.77%, LC90 = 10.86 mg/mL). In RCE, the presence of lectins and secondary metabolites: flavonoids, xanthones and phenols, were detected. The results demonstrate the potential of E. contortisiliquum seed extracts with ovicidal and larvicidal action on A. aegypti.
本研究评估了粗提物对埃及伊蚊卵和幼虫的杀虫活性，并验证了粗提物的植物化学特征和凝集素的存在。0.15 M NaCl 盐水溶液用作提取物质。对于卵和幼虫的测试，粗提物以其原始形式（RCE）和在 100°C 下煮沸 5 分钟（BCE）使用。浓度分别为 4.68；9.37；18.75；28.13；37.13 和 46.89 mg/mL，以蒸馏水作为阴性对照。一式三份进行测定。结果进行方差分析、Tukey 检验和 Log-Probit 分析，以确定 LC50 和 LC90。BCE 在卵上的效果优于 RCE，在 46.89 mg/mL 浓度下，能够阻止 81.66%±10.40%的处理卵孵化成幼虫。LC50 和 LC90 分别为 35.95 和 52.67 mg/mL。在幼虫测试中，浓度为 46.89 和 37.13 mg/mL 的 RCE 和 BCE 在暴露 24 小时内导致 100%死亡率。在其他浓度下，幼虫死亡率随着暴露时间延长至 48 小时而增加。在 48 小时暴露时，RCE 对幼虫最有前景（E=72.77%，LC90=10.86 mg/mL）。在 RCE 中，检测到凝集素和次级代谢物：类黄酮、蒽酮和酚类的存在。结果表明，E. contortisiliquum 种子提取物具有杀卵和杀幼虫活性，对埃及伊蚊有潜在的作用。
