Key Laboratory of Biological and Chemical Utilization of Zhejiang Forest Resources, Department of Forest Foods, Zhejiang Academy of Forestry, Hangzhou 310023, China.
Key Laboratory of Biological and Chemical Utilization of Zhejiang Forest Resources, Department of Forest Foods, Zhejiang Academy of Forestry, Hangzhou 310023, China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 7):127463. doi: 10.1016/j.ijbiomac.2023.127463. Epub 2023 Oct 16.
Variations in the structure and activities of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg fermented by Sanghuangporus sanghuang fungi were investigated. Compare with the unfermented polysaccharide (THDP2), the major monosaccharide composition and molecular weight of polysaccharide after fermentation (F-THDP2) altered dramatically, which caused galactose-induced conversion from glucose and one-third of molecular weight. F-THDP2 had a molecular weight of 1.23 × 10 Da. Moreover, the glycosidic linkage of F-THDP2 varied significantly, a 1, 2-linked α-d-Galp and 1, 2-linked α-d-Manp backbone was established in F-THDP2, which differed from that of 1, 4-linked α-d-Glcp and 1, 4-linked β-d-Galp in THDP2. In addition, F-THDP2 showed a more flexible chain conformation than that of THDP2 in aqueous solution. Strikingly, F-THDP2 exhibited superior inhibitory effects on HeLa cells via Fas/FasL-mediated Caspase-3 signaling pathways than that of the original polysaccharide. These variations in both structure and biological activities indicated that fermentation-mediated modification by Sanghuangporus sanghuang might a promising novel method for the effective conversion of starch and other polysaccharides from Tetrastigma hemsleyanum Diels et Gilg into highly bioactive biomacromolecules, which could be developed as a potential technology for use in the food industry.
研究了桑黄真菌发酵的玉竹多糖（THDP2）的结构和活性变化。与未发酵多糖（THDP2）相比，发酵后多糖（F-THDP2）的主要单糖组成和分子量发生了显著变化，导致半乳糖诱导的葡萄糖和三分之一的分子量转化。F-THDP2 的分子量为 1.23×10 Da。此外，F-THDP2 的糖苷键发生了显著变化，在 F-THDP2 中建立了 1,2-连接的α-d-Galp 和 1,2-连接的α-d-Manp 主链，这与 THDP2 中的 1,4-连接的α-d-Glcp 和 1,4-连接的β-d-Galp 不同。此外，F-THDP2 在水溶液中表现出比 THDP2 更灵活的链构象。值得注意的是，F-THDP2 通过 Fas/FasL 介导的 Caspase-3 信号通路对 HeLa 细胞的抑制作用优于原始多糖。这些结构和生物活性的变化表明，桑黄发酵介导的修饰可能是一种很有前途的方法，可以有效地将玉竹中的淀粉和其他多糖转化为具有高度生物活性的生物大分子，可作为食品工业中潜在的应用技术。
