U.S. Department of Agriculture, Agricultural Research Service, U.S. Horticultural Research Laboratory, 2001 South Rock Road, Fort Pierce, FL, USA.
Sci Rep. 2023 Oct 18;13(1):17807. doi: 10.1038/s41598-023-44832-x.
Commercial citrus trees are composed of a scion grafted onto a rootstock. Because grafting is one of the most expensive methods of plant propagation, grafting efficiency is of large practical importance. The purpose of this study was to improve citrus bud-grafting efficiency. The effects of six factors that included BA, Tween-20, DMSO, type of solvent (water or EtOH), cardinal orientation of grafted bud, and type of supplemental light (LED, metal halide, none) on forty-four bud-grafting measures were determined using a multifactor design of experiment approach. Four measures useful for identifying treatments of practical value included the number of rootstock axial buds that formed shoots, the percentage of grafted buds that formed shoots, the length of the longest shoot formed from the grafted buds, and the total leaf area of the grafted bud shoots. The factors that most affected these responses were no supplemental light to minimize the number of shoots from rootstock axial buds, a south orientation and 5 mM BA to maximize the percentage of grafted buds that formed shoots, a north orientation and 5 mM BA to maximize the length of the longest grafted bud shoot, and 5 mM BA to maximize the leaf area of the grafted bud shoots.
商业柑橘树由接穗嫁接到砧木上组成。由于嫁接是植物繁殖最昂贵的方法之一，因此嫁接效率具有重要的实际意义。本研究旨在提高柑橘芽接效率。采用多因素试验设计方法，确定了 6 个因素（BA、吐温-20、DMSO、溶剂类型（水或 EtOH）、嫁接芽的方位和补充光类型（LED、金属卤化物、无））对 44 个芽接措施的影响。四项有助于识别具有实用价值的处理方法的措施包括形成芽的砧木轴向芽的数量、形成芽的嫁接芽的百分比、从嫁接芽形成的最长芽的长度以及嫁接芽芽的总叶面积。对这些反应影响最大的因素是没有补充光照以最小化砧木轴向芽的芽数，南向和 5 mM BA 以最大化形成芽的嫁接芽的百分比，北向和 5 mM BA 以最大化最长嫁接芽的长度，以及 5 mM BA 以最大化嫁接芽芽的叶面积。
