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基于荷电液滴的胆汁酸衍生化在循环离子淌度质谱平台上的实现。

Implementation of charged microdroplet-based derivatization of bile acids on a cyclic ion mobility spectrometry-mass spectrometry platform.

机构信息

Department of Chemistry, University of Utah, 315 South 1400 East, Room 2020, Salt Lake City, Utah 84112, USA.

出版信息

Anal Methods. 2023 Nov 2;15(42):5577-5581. doi: 10.1039/d3ay01447a.

Abstract

Herein, we report the first implementation of charged microdroplet-based derivatization on a commercially-available cyclic ion mobility spectrometry-mass spectrometry platform. We have demonstrated the potential of our approach to improve separability of challenging isomers, but more importantly to rapidly screen derivatization reactions through droplet chemistry. Additionally, the use of cyclic ion mobility separations and tandem mass spectrometry reveals insights into product formation that would be lost with single stage mass spectrometry. Overall, we anticipate broad utility of our methodology owing to the simple design and setup for performing these droplet-based reactions and future work coupling these reactions online with liquid chromatography.

摘要

在这里,我们首次在市售的循环离子淌度谱-质谱联用平台上实现了荷电微滴衍生化。我们已经证明了我们的方法有潜力提高具有挑战性的异构体的分离度,但更重要的是,通过液滴化学快速筛选衍生化反应。此外,循环离子淌度分离和串联质谱的使用揭示了产物形成的一些见解,如果仅使用单级质谱,则可能会丢失这些见解。总的来说,我们预计我们的方法具有广泛的应用前景,因为这种基于液滴的反应的设计和设置简单,并且未来可以将这些反应与液相色谱在线耦合。

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