Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Division of Laboratory Medicine, King Chulalongkorn Memorial Hospital, Bangkok, Thailand.
Clin Chem Lab Med. 2023 Oct 20;62(4):690-697. doi: 10.1515/cclm-2023-1000. Print 2024 Mar 25.
Automated hematology analysis is expected to improve the performance of platelet counting. We evaluated the performance of a new platelet counting, hybrid (PLT-H) and also impedance (PLT-I) and optical (PLT-O) on the BC-780 automated hematology analyzer compared to the international reference method (IRM) in blood samples with thrombocytopenic and platelet interference.
The basic platelet count performance of the BC-780 automated hematology analyzer was evaluated according to the requirements of the Clinical Laboratory and Standards Institute (CLSI) Document H26-A2. Additionally, the thrombocytopenic (low PLT count) blood samples and the platelet interference blood samples including fragmented red blood cells (RBCs), microcytes or small RBCs, and giant platelets were determined with the BC-780 hematology analyzer compared to the IRM.
Blank counting and the carry-over contamination rate of platelet count using the BC-780 both met the manufacturers' claim. For both 123 thrombocytopenic and 232 platelet interference blood samples (72 fragmented RBCs, 91 microcytes and 51 giant platelets), all three platelet counting methods exhibited high comparability with the IRM (the lowest correlation (r)=0.916). Interestingly, the comparability of PLT-H (r=0.928-0.986) with the IRM was better than that of PLT-I (r=0.916-0.979).
The performance of PLT-H in the BC-780 met the manufacturer's specifications. PLT-H exhibits better reproducibility than did PLT-I, correlates well with the PLT-O for thrombocytopenic samples and demonstrates good anti-interference ability. PLT-H counting is therefore recommended as a zero-cost alternative platelet counting method for platelet interference samples in clinical settings.
自动化血液分析有望提高血小板计数的性能。我们评估了新型血小板计数方法，即混合（PLT-H）和阻抗（PLT-I）以及光学（PLT-O）在血小板减少和血小板干扰的血液样本上与国际参考方法（IRM）相比在 BC-780 自动化血液分析仪上的性能。
根据临床实验室和标准协会（CLSI）文件 H26-A2 的要求，评估了 BC-780 自动化血液分析仪的基本血小板计数性能。此外，与 IRM 相比，使用 BC-780 血液分析仪确定了血小板减少（低血小板计数）血液样本和血小板干扰血液样本，包括碎片红细胞（RBC）、微细胞或小 RBC 和巨大血小板。
空白计数和使用 BC-780 的血小板计数的携带污染率均符合制造商的要求。对于 123 例血小板减少和 232 例血小板干扰血液样本（72 个碎片 RBC、91 个微细胞和 51 个巨大血小板），所有三种血小板计数方法与 IRM 具有高度可比性（最低相关性（r）=0.916）。有趣的是，PLT-H（r=0.928-0.986）与 IRM 的可比性优于 PLT-I（r=0.916-0.979）。
BC-780 中 PLT-H 的性能符合制造商的规格。PLT-H 比 PLT-I 具有更好的可重复性，与血小板减少样本的 PLT-O 相关性良好，并具有良好的抗干扰能力。因此，建议将 PLT-H 计数作为血小板干扰样本在临床环境中的零成本替代血小板计数方法。
