College of Veterinary Medicine, Northeast Agricultural University , Harbin, China.
College of Food Science, Northeast Agricultural University , Harbin, China.
Microbiol Spectr. 2023 Dec 12;11(6):e0245523. doi: 10.1128/spectrum.02455-23. Epub 2023 Oct 19.
Avian coccidiosis caused by brings huge economic losses to the poultry industry. Although live vaccines and anti-coccidial drugs were used for a long time, infection in chicken farms all over the world commonly occurred. The exploration of novel, effective vaccines has become a research hotspot. parasites have complex life cycles, and effective antigens are particularly critical to developing anti-coccidial vaccines. Microneme proteins (MICs), secreted from microneme organelles located at the parasite apex, are considered immunodominant antigens. microneme 3 (EtMIC3) contains four conserved repeats (MARc1, MARc2, MARc3, and MARc4) and three divergent repeats (MARa, MARb, and MARd), which play a vital role during the invasion. is a native probiotic in animal intestines and can regulate intestinal flora. In this study, BC1 and C4D domains of EtMIC3, BC1 or C4D fusing to dendritic cells targeting peptides, were surface-displyed by respectively. Oral immunizations were performed to investigate immune protective effects against infection.
由 引起的禽类球虫病给家禽养殖业带来了巨大的经济损失。尽管长期以来一直使用活疫苗和抗球虫药物，但世界各地的养鸡场仍普遍发生 感染。探索新型、有效的疫苗已成为研究热点。 寄生虫具有复杂的生命周期，有效的抗原对于开发抗球虫疫苗至关重要。微线蛋白（MICs）从位于寄生虫顶端的微线细胞器中分泌出来，被认为是免疫优势抗原。 微线蛋白 3（EtMIC3）包含四个保守重复（MARc1、MARc2、MARc3 和 MARc4）和三个发散重复（MARa、MARb 和 MARd），在 入侵过程中发挥着重要作用。 是动物肠道中的一种天然益生菌，能够调节肠道菌群。在这项研究中，通过 分别表面展示了 EtMIC3 的 BC1 和 C4D 结构域，以及与树突状细胞靶向肽融合的 BC1 或 C4D。通过口服免疫来研究其对 感染的免疫保护作用。
