Wang X R, Li S, Liu J Y, Gu B, Jia Z J, Tang B
Medical Technology School of Xuzhou Medical University, Xuzhou 221004, China.
Nanjing Vazyme Medical Technology Co., Ltd, Nanjing 210000, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2023 Oct 6;57(10):1608-1612. doi: 10.3760/cma.j.cn112150-20230411-00280.
This study aims to develop a rapid and convenient test card for simultaneous detection of influenza A and influenza B viruses using quantum dot-based immunochromatographic assay. The test card consists of a test strip and a plastic casing. The test strip is composed of absorbent paper, a buffer pad, nitrocellulose membrane (NC membrane), sample pad, quantum dot-labeled antibody pad, and polyvinyl chloride (PVC) board. The NC membrane is coated with mouse monoclonal antibodies against influenza A and influenza B viruses for the T lines (test lines), and reference proteins A and B for the C line (control line). The quantum dot-labeled antibody pad contains mouse monoclonal antibody-quantum dot conjugates against influenza A and influenza B viruses. The results showed that the detection limit of the test card for both viruses ranged from 1.51 ×10 to 2.71×10 TCID50/ml, indicating its sensitivity for accurate detection of influenza A and influenza B viruses without being affected by various variants. The test card exhibited specific reactions with different subtypes of influenza A and influenza B virus culture fluids and showed no cross-reactivity with adenovirus, novel coronavirus, Mycoplasma pneumoniae, respiratory syncytial virus, Staphylococcus aureus, and other pathogens. Overall, the sensitivity and specificity of the test card for simultaneous detection of influenza A and influenza B viruses meet the requirements for clinical use. It offers the advantages of simplicity, rapidity, and no requirement for special equipment, enabling quick auxiliary diagnosis to prevent disease transmission.
本研究旨在开发一种基于量子点免疫层析法的快速便捷检测卡，用于同时检测甲型和乙型流感病毒。该检测卡由试纸条和塑料外壳组成。试纸条由吸水纸、缓冲垫、硝酸纤维素膜（NC膜）、样品垫、量子点标记抗体垫和聚氯乙烯（PVC）板组成。NC膜上包被有针对甲型和乙型流感病毒的鼠单克隆抗体作为T线（检测线），以及用于C线（对照线）的参考蛋白A和B。量子点标记抗体垫包含针对甲型和乙型流感病毒的鼠单克隆抗体 - 量子点偶联物。结果表明，该检测卡对两种病毒的检测限为1.51×10至2.71×10 TCID50/ml，表明其对准确检测甲型和乙型流感病毒具有敏感性，且不受各种变异体的影响。该检测卡与甲型和乙型流感病毒不同亚型的培养液呈现特异性反应，与腺病毒、新型冠状病毒、肺炎支原体、呼吸道合胞病毒、金黄色葡萄球菌等其他病原体无交叉反应。总体而言，该检测卡同时检测甲型和乙型流感病毒的敏感性和特异性符合临床使用要求。它具有操作简单、快速且无需特殊设备的优点，能够实现快速辅助诊断以预防疾病传播。
