Gattoni Giuliano, Di Costanzo Fabiana, de la Haba Rafael R, Fernández Ana B, Guerrero-Flores Shaday, Selem-Mojica Nelly, Ventosa Antonio, Corral Paulina
Department of Biology, University of Naples Federico II, Naples, Italy.
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, Sevilla, Spain.
Front Microbiol. 2023 Sep 29;14:1238779. doi: 10.3389/fmicb.2023.1238779. eCollection 2023.
Shifting the bioprospecting targets toward underexplored bacterial groups combined with genome mining studies contributes to avoiding the rediscovery of known compounds by revealing novel, promising biosynthetic gene clusters (BGCs). With the aim of determining the biosynthetic potential of a novel marine bacterium, strain V10, isolated from the Domitian littoral in Italy, a comparative phylogenomic mining study was performed across related photosynthetic bacterial groups from an evolutionary perspective. Studies on polyphasic and taxogenomics showed that this bacterium constitutes a new species, designated sp. nov. To date, this genus has only one other validly described species, which was isolated from a hypersaline Antarctic lake. The genomic evolutionary study linked to BGC diversity revealed that there is a close relationship between the phylogenetic distance of the members of the photosynthetic genera , and and their BGC profiles, whose conservation pattern allows discriminating between these genera. On the contrary, the rest of the species related to exhibited an individual species pattern unrelated to genome size or source of isolation. This study showed that photosynthetic strains possess a streamlined content of BGCs, of which 94.34% of the clusters with biotechnological interest (NRPS, PKS, RRE, and RiPP) are completely new. Among these stand out T1PKS, exclusive of V10, and RRE, highly conserved only in V10 and , both categories of BGCs involved in the synthesis of plant growth-promoting compounds and antitumoral compounds, respectively. In all cases, with very low homology with already patented molecules. Our findings reveal the high biosynthetic potential of infrequently cultured bacterial groups, suggesting the need to redirect attention to microbial minorities as a novel and vast source of bioactive compounds still to be exploited.
将生物勘探目标转向未充分探索的细菌群体，并结合基因组挖掘研究，有助于通过揭示新的、有前景的生物合成基因簇（BGC）来避免已知化合物的重新发现。为了确定从意大利多米提安海岸分离出的新型海洋细菌V10菌株的生物合成潜力，从进化角度对相关光合细菌群体进行了比较系统基因组挖掘研究。多相和分类基因组学研究表明，这种细菌构成了一个新物种，命名为 sp. nov. 迄今为止，该属只有另一个有效描述的物种，是从南极高盐湖泊中分离出来的。与BGC多样性相关的基因组进化研究表明，光合属 、 和 的成员之间的系统发育距离与其BGC图谱之间存在密切关系，其保守模式允许区分这些属。相反，与 相关的其他物种表现出与基因组大小或分离来源无关的个体物种模式。这项研究表明，光合菌株拥有精简的BGC含量，其中94.34%具有生物技术意义的簇（NRPS、PKS、RRE和RiPP）是全新的。其中突出的是仅V10特有的T1PKS和仅在V10和 中高度保守的RRE，这两类BGC分别参与植物生长促进化合物和抗肿瘤化合物的合成。在所有情况下，与已获专利的分子同源性都非常低。我们的研究结果揭示了未经常培养的细菌群体具有很高的生物合成潜力，这表明有必要将注意力重新转向微生物小众群体，将其作为仍有待开发的新型丰富生物活性化合物来源。
