Shen Jie, Wang Xi, Song Huifang, Wang Mingyang, Niu Tianzeng, Lei Haiying, Qin Cheng, Liu Ake
Department of Life Sciences, Changzhi University, Changzhi 046011, China.
School of Life Science, Shanxi Normal University, Taiyuan 030031, China.
iScience. 2023 Oct 2;26(11):108112. doi: 10.1016/j.isci.2023.108112. eCollection 2023 Nov 17.
Drought can adversely influence the crop growth and production. Accordingly, sunflowers have strong adaptability to drought; hence, we conducted analyses for sunflower seedlings with drought stress and rehydration drought acclimation through physiological measurements and transcriptomics. It showed that drought can cause the accumulation of ROS and enhance the activity of antioxidant enzymes and the content of osmolytes. After rehydration, the contents of ROS and MDA were significantly reduced concomitant with increased antioxidant activity and osmotic adjustment. Totally, 2,589 DEGs were identified among treatments. Functional enrichment analysis showed that DEGs were mainly involved in plant hormone signal transduction, MAPK signaling, and biosynthesis of secondary metabolites. Comparison between differentially spliced genes and DEGs indicated that , , and may be pivotal genes involved in sunflower drought resistance. Our results not only highlight the underlying mechanism of drought stress and rehydration in sunflower but also provide a theoretical basis for crop genetic breeding.
干旱会对作物生长和产量产生不利影响。因此，向日葵对干旱具有较强的适应性；因此，我们通过生理测量和转录组学对遭受干旱胁迫和复水干旱驯化的向日葵幼苗进行了分析。结果表明，干旱会导致活性氧（ROS）积累，增强抗氧化酶活性和渗透调节物质含量。复水后，ROS和丙二醛（MDA）含量显著降低，同时抗氧化活性和渗透调节增强。各处理间共鉴定出2589个差异表达基因（DEGs）。功能富集分析表明，DEGs主要参与植物激素信号转导、丝裂原活化蛋白激酶（MAPK）信号传导和次生代谢物生物合成。差异剪接基因与DEGs的比较表明， 、 和 可能是参与向日葵抗旱的关键基因。我们的研究结果不仅揭示了向日葵干旱胁迫和复水的潜在机制，也为作物遗传育种提供了理论依据。
