一种基于微电穿孔/电泳的疫苗筛选系统揭示了接种顺序对交叉保护性免疫的影响。
A micro-electroporation/electrophoresis-based vaccine screening system reveals the impact of vaccination orders on cross-protective immunity.
作者信息
Li Yongyong, Mo Jingshan, Liu Jing, Liang Ying, Deng Caiguanxi, Huang Zhangping, Jiang Juan, Liu Ming, Liu Xinmin, Shang Liru, Wang Xiafeng, Xie Xi, Wang Ji
机构信息
Division of Pulmonary and Critical Care Medicine, Institute of Precision Medicine, The First Affiliated Hospital of Sun Yat-sen University, Institute of Respiratory Diseases of Sun Yat-sen University, Sun Yat-sen University, Guangzhou 510080, Peoples Republic of China.
State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou 510006, People's Republic of China.
出版信息
iScience. 2023 Sep 28;26(10):108086. doi: 10.1016/j.isci.2023.108086. eCollection 2023 Oct 20.
The constant emergence of mutated pathogens poses great challenges to the existing vaccine system. A screening system is needed to screen for antigen designs and vaccination strategies capable of inducing cross-protective immunity. Herein, we report a screening system based on DNA vaccines and a micro-electroporation/electrophoresis system (MEES), which greatly improved the efficacy of DNA vaccines, elevating humoral and cellular immune responses by over 400- and 35-fold respectively. Eighteen vaccination strategies were screened simultaneously by sequential immunization with vaccines derived from wildtype (WT) SARS-CoV-2, Delta, or Omicron BA.1 variant. Sequential vaccination of BA.1-WT-Delta vaccines with MEES induced potent neutralizing antibodies against all three viral strains and BA.5 variant, demonstrating that cross-protective immunity against future mutants can be successfully induced by existing strain-derived vaccines when a proper combination and order of sequential vaccination are used. Our screening system could be used for fast-seeking vaccination strategies for emerging pathogens in the future.
突变病原体的不断出现对现有的疫苗系统构成了巨大挑战。需要一个筛选系统来筛选能够诱导交叉保护性免疫的抗原设计和疫苗接种策略。在此，我们报告了一种基于DNA疫苗和微电穿孔/电泳系统（MEES）的筛选系统，该系统极大地提高了DNA疫苗的效力，使体液免疫和细胞免疫反应分别提高了400倍和35倍以上。通过用源自野生型（WT）SARS-CoV-2、Delta或Omicron BA.1变体的疫苗进行序贯免疫，同时筛选了18种疫苗接种策略。用MEES对BA.1-WT-Delta疫苗进行序贯接种可诱导针对所有三种病毒株和BA.5变体的强效中和抗体，这表明当采用适当的序贯接种组合和顺序时，现有毒株衍生的疫苗可以成功诱导针对未来突变体的交叉保护性免疫。我们的筛选系统可用于未来快速寻找针对新兴病原体的疫苗接种策略。
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