Department of Biomedical Science, Translational Research center, Inha University Hospital, Incheon, Korea.
Inha Institute of Aerospace Medicine, Inha University College of Medicine, Incheon, Korea.
Technol Cancer Res Treat. 2023 Jan-Dec;22:15330338221147122. doi: 10.1177/15330338221147122.
Irreversible electroporation (IRE) is a non-thermal and minimal invasive modality to ablate pathologic lesions such as hepatic tumors. Histological analysis of the initial lesions after IRE can help predict ablation efficacy. We aimed to investigate the histological characteristics of early hepatic lesions after IRE application using animal models. IRE (1500 V/cm, a pulse length of 100 s, 60 or 90 pulses) was applied to the liver of miniature pigs. H&E and TUNEL staining were performed and analyzed. Ablated zones of pig liver were discolored and separated from the normal zone after IRE. Histologic characteristics of ablation zones included preserved hepatic lobular architecture with a unique hexagonal-like structure. Apoptotic cells were detected, and sinusoidal dilatation and blood congestion were observed, but hepatic arteries and bile ducts were intact around the ablation zones. The early lesions obtained by delivering monophasic square wave pulses through needle electrodes reflected typical histological changes induced by IRE. Therefore, it was found that the histological assessment of the early hepatic lesion after IRE can be utilized to predict the IRE ablation effect.
不可逆电穿孔（IRE）是一种非热和微创的方法，可消融肝肿瘤等病变。IRE 后初始病变的组织学分析有助于预测消融效果。我们旨在使用动物模型研究 IRE 应用后早期肝损伤的组织学特征。IRE（1500V/cm，脉冲长度 100s，60 或 90 个脉冲）应用于小型猪的肝脏。进行 H&E 和 TUNEL 染色并进行分析。IRE 后，猪肝脏的消融区变色并与正常区分离。消融区的组织学特征包括保留的肝小叶结构，具有独特的六边形结构。检测到凋亡细胞，观察到窦扩张和充血，但消融区周围的肝动脉和胆管完整。通过针状电极传递单相方波脉冲获得的早期病变反映了 IRE 诱导的典型组织学变化。因此，发现 IRE 后早期肝损伤的组织学评估可用于预测 IRE 消融效果。
