Key Laboratory of Agri-Products Quality and Biosafety, Ministry of Education, Anhui Provincial Key Laboratory of Integrated Pest Management on Crops, School of Plant Protection, Anhui Agricultural University, Hefei, China.
Sichuan Branch of China National Tobacco Corporation, Chengdu, China.
Pest Manag Sci. 2024 Mar;80(3):1107-1117. doi: 10.1002/ps.7841. Epub 2023 Nov 8.
Chlorantraniliprole (CAP) is a diamide insecticide with high efficacy against many pest insects, including the black cutworm, Agrotis ipsilon. Agrotis ipsilon is a serious pest causing significant yield losses in crops. Glutathione-S-transferases (GSTs) belong to a family of metabolic enzymes that can detoxify a wide range of pesticides. However, little is known about the functions of GSTs in CAP tolerance in A. ipsilon.
A cDNA sequence (designated AiGSTu1) encoding an unclassified GST was identified from A. ipsilon. AiGSTu1 is highly expressed during the 3 -instar larval and the pupal stages. Most of the mRNA transcripts were found in larval Malpighian tubules. Exposure to CAP strongly enhanced AiGSTu1 expression, GST activity, hydrogen peroxide (H O ) and malondialdehyde levels in larvae. H O treatment upregulated the transcription level of AiGSTu1, suggesting that CAP-induced oxidative stress may activate AiGSTu1 expression. The activity of recombinant AiGSTu1 was inhibited by CAP in a dose-dependent manner. Metabolism assay results demonstrated that AiGSTu1 is capable of depleting CAP. Overexpression of AiGSTu1 enhanced the tolerance of Escherichia coli cells to H O and the oxidative stress inducer, cumene hydroperoxide. Silencing of AiGSTu1 by RNA interference increased the susceptibility of A. ipsilon larvae to CAP.
The findings of this study provide valuable insights into the potential role of AiGSTu1 in CAP detoxification and will improve our understanding of CAP tolerance in A. ipsilon. © 2023 Society of Chemical Industry.
氯虫苯甲酰胺（CAP）是一种高效的二酰胺类杀虫剂，对包括斜纹夜蛾（Agrotis ipsilon）在内的许多害虫都有很好的防治效果。斜纹夜蛾是一种严重的害虫，会导致农作物产量显著下降。谷胱甘肽-S-转移酶（GSTs）属于代谢酶家族，能够解毒多种农药。然而，关于 GSTs 在斜纹夜蛾对 CAP 耐受中的作用知之甚少。
从斜纹夜蛾中鉴定出一个编码未分类 GST 的 cDNA 序列（命名为 AiGSTu1）。AiGSTu1 在 3 龄幼虫和蛹期表达水平较高。大部分 mRNA 转录本存在于幼虫的马氏管中。暴露于 CAP 强烈增强了 AiGSTu1 的表达、GST 活性、过氧化氢（H₂O₂）和丙二醛水平在幼虫中。H₂O₂处理上调了 AiGSTu1 的转录水平，表明 CAP 诱导的氧化应激可能激活了 AiGSTu1 的表达。CAP 以剂量依赖的方式抑制重组 AiGSTu1 的活性。代谢测定结果表明 AiGSTu1 能够耗尽 CAP。AiGSTu1 的过表达增强了大肠杆菌细胞对 H₂O₂和氧化应激诱导剂 cumene hydroperoxide 的耐受能力。通过 RNA 干扰沉默 AiGSTu1 增加了斜纹夜蛾幼虫对 CAP 的敏感性。
本研究结果为 AiGSTu1 在 CAP 解毒中的潜在作用提供了有价值的见解，并将提高我们对斜纹夜蛾对 CAP 耐受的理解。© 2023 化学工业协会。
