接触有毒铜水平后，血浆和红细胞膜脂肪酸组成的改变会影响雌性 Wistar 大鼠的膜变形性和流动性。

Alterations in plasma and erythrocyte membrane fatty acid composition following exposure to toxic copper level affect membrane deformability and fluidity in female wistar rats.

机构信息

Applied and Environmental Physiology Unit, Department of Physiology, Faculty of Basic Medical Sciences, University of Ibadan, Nigeria.

出版信息

J Trace Elem Med Biol. 2023 Dec;80:127316. doi: 10.1016/j.jtemb.2023.127316. Epub 2023 Oct 5.

DOI:10.1016/j.jtemb.2023.127316

PMID:37862897

Abstract

BACKGROUND

Deformability and fluidity function of the red blood cell membrane are properties defined by the lipid composition. Toxic copper level induces membrane lipid peroxidation which could cause membrane instability. This study therefore investigated the effect of exposure to toxic copper level for 30 days on red blood cell membrane deformability and fluidity in female Wistar rats.

METHODS

Twelve (12) female Wistar rats (160 ± 10 g) were randomly grouped (n = 6) into control (given 0.1 ml distilled water p.o.) and copper-toxic (100 mg/kg Copper Sulphate, p.o.), and treated for 30 days. Plasma obtained and RBC membrane prepared from blood collected over EDTA post-treatment were assayed for total cholesterol (TC), phospholipids and fatty acid profile using spectrophotometry and Gas chromatography while heparinized blood was subjected to fragility test. Data were analyzed using student T-test for statistical significance at p < 0.05.

RESULTS AND CONCLUSION

Plasma TC increased by 4.33% while RBC membrane TC decreased by 20.32% in copper-toxic group compared to control. Compared to control, excess copper significantly increased membrane phospholipids level (0.72 ± 0.01 vs 0.59 ± 0.04 mg/dL) but reduced membrane cholesterol/phospholipid ratio (46.61 ± 4.72 vs 72.66 ± 6.47) and stability (by 23.53%). Number of cis- and saturated fatty acids increased in copper-treated plasma and RBC membrane compared to control. Exposure to toxic copper level alters erythrocyte membrane fluidity and deformability by disrupting membrane lipid composition, saturation, bond configuration in phospholipids and permeability.

摘要

背景

红细胞膜的变形性和流动性功能是由脂质组成决定的特性。毒性铜水平诱导膜脂质过氧化，可能导致膜不稳定。因此，本研究调查了暴露于毒性铜水平 30 天对雌性 Wistar 大鼠红细胞膜变形性和流动性的影响。

方法

将 12 只（12 只）雌性 Wistar 大鼠（160 ± 10 g）随机分为对照组（给予 0.1 ml 蒸馏水口服）和铜中毒组（100 mg/kg 硫酸铜，口服），并治疗 30 天。在治疗后用 EDTA 收集的血液中获得血浆并从红细胞膜中提取并使用分光光度法和气相色谱法测定总胆固醇（TC）、磷脂和脂肪酸谱，肝素化血液进行脆性试验。数据使用学生 t 检验进行分析，以 p < 0.05 表示统计学意义。

结果和结论

与对照组相比，铜中毒组血浆 TC 增加了 4.33%，而 RBC 膜 TC 降低了 20.32%。与对照组相比，过量的铜显著增加了膜磷脂水平（0.72 ± 0.01 对 0.59 ± 0.04 mg/dL），但降低了膜胆固醇/磷脂比（46.61 ± 4.72 对 72.66 ± 6.47）和稳定性（降低 23.53%）。与对照组相比，铜处理的血浆和 RBC 膜中的顺式和饱和脂肪酸数量增加。暴露于毒性铜水平通过破坏膜脂质组成、饱和度、磷脂中的键构象和通透性来改变红细胞膜的流动性和变形性。