School of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China.
School of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China.
Food Chem. 2024 Mar 15;436:137769. doi: 10.1016/j.foodchem.2023.137769. Epub 2023 Oct 15.
Meat freshness is related to food safety and human health. Developing a simple and effective method for on-site detection of meat freshness is essential to ensure food safety. This study aimed to explore a ratiometric fluorescence platform for on-site screening of meat freshness. We synthesized a series of benzothiazole-based fluorescent compounds (BM, BHM and BTH), each with different recognition groups for detecting meat freshness biomarkers cadaverine (Cad) and putrescine (Pte). The optimized 2-(2'-hydroxyphenyl-3-aldehyde-5-1,3-indanedione) benzothiazole (BTH) demonstrated a noticeable color and fluorescence change, a fast response (<15 min), and high selectivity and sensitivity (LOD = 70 nM) to Cad. Portable test strips based on BTH were prepared for rapid visual detection of meat freshness, which exhibited visible color and fluorescen color changes to Cad and Pte. Furthermore, a portable smartphone-based fluorescence device integrated with a self-programmed Python program was fabricated and used on-site to monitor Cad and Pte within 5 min. The BTH-loaded portable test strips were successfully employed as low-cost, high-contrast, fast-response, and smartphone-adaptable fluorescent labels for detecting Cad and Pte in meat samples under different temperatures (25 °C, 4 °C, and -20 °C). This enabled consumers and food supply chain stakeholders to quickly and visually monitor the meat freshness in real beef, chicken, and pork products.
肉的新鲜度与食品安全和人类健康息息相关。开发一种简单有效的现场检测肉类新鲜度的方法对于确保食品安全至关重要。本研究旨在探索一种用于现场筛选肉类新鲜度的比率荧光平台。我们合成了一系列苯并噻唑基荧光化合物（BM、BHM 和 BTH），每个化合物都具有不同的识别基团，用于检测肉类新鲜度生物标志物尸胺（Cad）和腐胺（Pte）。优化后的 2-（2'-羟基苯-3-醛-5-1,3-茚二酮）苯并噻唑（BTH）表现出明显的颜色和荧光变化，快速响应（<15 分钟），对 Cad 具有高选择性和灵敏度（LOD=70 nM）。基于 BTH 的便携式测试条用于快速视觉检测肉类新鲜度，对 Cad 和 Pte 显示出可见的颜色和荧光颜色变化。此外，我们还制备了一种基于智能手机的便携式荧光装置，集成了自编的 Python 程序，并在现场用于监测 5 分钟内 Cad 和 Pte 的变化。BTH 负载的便携式测试条成功用作低成本、高对比度、快速响应和智能手机适配的荧光标记物，用于检测不同温度（25°C、4°C 和-20°C）下肉样中的 Cad 和 Pte。这使得消费者和食品供应链利益相关者能够快速直观地监测真实牛肉、鸡肉和猪肉产品中的肉新鲜度。
