Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
Linong Testing (Binzhou) Co., Ltd., Binzhou Bohai Advanced Technology Research Institute, Binzhou, 256600, China.
Fish Shellfish Immunol. 2023 Dec;143:109180. doi: 10.1016/j.fsi.2023.109180. Epub 2023 Oct 19.
Polyascus gregaria, a parasitic barnacle, poses a significant threat to Eriocheir sinensis farms by inhibiting crab growth. However, the molecular and pathological mechanisms behind P. gregaria infection in the hepatopancreas of E. sinensis remain unclear. In this study, we investigated the impact and underlying mechanisms of P. gregaria infection on E. sinensis through analyzing the infected hepatopancreatic tissues by tandem mass tag technology and RNA-Seq high-throughput sequencing. Among the identified 10,693 differentially expressed genes, 294 genes were significantly altered following P. gregaria infection, including 92 upregulated and 202 downregulated genes. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses further revealed the involvement of these genes in oxidative decomposition, lipid metabolism, inflammation, and hepatopancreas metabolism. Meanwhile, the identified 253 differentially expressed proteins, including 143 upregulated and 110 downregulated proteins, are mainly related to cellular and metabolic processes, catalytic activity, and cell components. The pathway analysis indicated their enrichment in glycolysis/gluconeogenesis, oxidative phosphorylation, endoplasmic reticulum protein processing, and actin cytoskeleton regulation. The involvement of these differentially expressed genes and proteins in the peroxisome proliferator-activated receptors pathway during host immune responses against P. gregaria infection has been highlighted. Furthermore, pathological examinations and biochemical indicators jointly demonstrated the hepatopancreatic damage and increased oxidative stress and apoptosis in the infected E. sinensis. Collectively, our study provides crucial insights into the mechanisms underlying the E. sinensis-P. gregaria interactions, and may contribute to the development of novel strategies for parasite control and reducing economic losses in aquaculture.
聚缩虫是一种寄生藤壶，它通过抑制螃蟹的生长，对中华绒螯蟹养殖场构成了重大威胁。然而，聚缩虫感染中华绒螯蟹肝胰腺的分子和病理机制尚不清楚。在这项研究中，我们通过串联质量标签技术和 RNA-Seq 高通量测序分析感染的肝胰腺组织，研究了聚缩虫感染对中华绒螯蟹的影响及其潜在机制。在鉴定的 10693 个差异表达基因中，有 294 个基因在聚缩虫感染后发生了显著变化，包括 92 个上调基因和 202 个下调基因。基因本体论和京都基因与基因组百科全书分析进一步揭示了这些基因参与氧化分解、脂质代谢、炎症和肝胰腺代谢。同时，鉴定的 253 个差异表达蛋白，包括 143 个上调蛋白和 110 个下调蛋白，主要与细胞和代谢过程、催化活性和细胞成分有关。通路分析表明，它们在糖酵解/糖异生、氧化磷酸化、内质网蛋白加工和肌动蛋白细胞骨架调节中富集。宿主免疫反应中这些差异表达基因和蛋白在过氧化物酶体增殖物激活受体途径中的参与，突显了宿主对聚缩虫感染的免疫反应机制。此外，病理检查和生化指标共同证明了感染中华绒螯蟹的肝胰腺损伤以及氧化应激和细胞凋亡增加。总之，我们的研究为中华绒螯蟹-聚缩虫相互作用的机制提供了重要的见解，并可能有助于开发新的寄生虫控制策略，减少水产养殖的经济损失。
