College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Gene. 2024 Jan 30;893:147912. doi: 10.1016/j.gene.2023.147912. Epub 2023 Oct 18.
Heat shock proteins (HSPs) are essential for plant growth, development, and stress adaptation. However, their roles in Jerusalem artichoke are largely unexplored. Using bioinformatics, we classified 143 HSP genes into distinct families: HSP40 (82 genes), HSP60 (22 genes), HSP70 (29 genes), HSP90 (6 genes), and HSP100 (4 genes). Our analysis covered their traits, evolution, and structures. Using RNA-seq data, we uncovered unique expression patterns of these HSP genes across growth stages and tissues. Notably, HSP40, HSP60, HSP70, HSP90, and HSP100 families each had specific roles. We also studied how these gene families responded to various stresses, from extreme temperatures to drought and salinity, revealing intricate expression dynamics. Remarkably, HSP40 showed remarkable flexibility, while HSP60, HSP70, HSP90, and HSP100 responded specifically to stress types. Moreover, our analysis unveiled significant correlations between gene pairs under stress, implying cooperative interactions. qRT-PCR validation underscored the significance of particular genes such as HtHSP60-7, HtHSP90-5, HtHSP100-2, and HtHSP100-3 in responding to stress. In summary, our study advances the understanding of how HSP gene families collectively manage stresses in Jerusalem artichoke. This provides insights into specific gene functions and broader plant stress responses.
热休克蛋白（HSPs）对植物的生长、发育和应激适应至关重要。然而，它们在菊芋中的作用在很大程度上尚未被探索。我们使用生物信息学方法将 143 个 HSP 基因分为不同的家族：HSP40（82 个基因）、HSP60（22 个基因）、HSP70（29 个基因）、HSP90（6 个基因）和 HSP100（4 个基因）。我们的分析涵盖了它们的特征、进化和结构。使用 RNA-seq 数据，我们揭示了这些 HSP 基因在不同生长阶段和组织中的独特表达模式。值得注意的是，HSP40、HSP60、HSP70、HSP90 和 HSP100 家族各自具有特定的功能。我们还研究了这些基因家族如何响应各种应激，从极端温度到干旱和盐度，揭示了复杂的表达动态。值得注意的是，HSP40 表现出显著的灵活性，而 HSP60、HSP70、HSP90 和 HSP100 则对特定的应激类型作出反应。此外，我们的分析揭示了在应激下基因对之间存在显著相关性，暗示了协同相互作用。qRT-PCR 验证强调了特定基因如 HtHSP60-7、HtHSP90-5、HtHSP100-2 和 HtHSP100-3 在应对应激中的重要性。总之，我们的研究增进了对 HSP 基因家族如何共同管理菊芋中应激的理解。这为特定基因功能和更广泛的植物应激反应提供了深入了解。
