Ministry of Education-Shanghai Key Laboratory of Children's Environmental Health, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Obstetrics and Gynecology, Shanghai Changhai Hospital, Shanghai, China.
Cryobiology. 2023 Dec;113:104786. doi: 10.1016/j.cryobiol.2023.104786. Epub 2023 Oct 18.
Cryopreservation is widely used for the long-term storage of bacteria. Glycerol is one of the traditional cryoprotectants used widely to prevent cryoinjury during the cryopreservation of bacteria,although it may be toxic to the cells. To overcome these issues, synthetic antifreeze polymers are also used as cryoprotectants to inhibit ice formation. In the study, we compared the performance of various antifreeze synthetic polymers including poly(vinyl alcohol) (PVA), poly(vinylpyrrolidone), poly(ethylene glycol), and dextran with glycerol, among which PVA performed best on decreasing the ice growth rate.The impacts of glycerol, trehalose, combined with PVA on the survival of S. thermophilus were also explored. Notably,. S. thermophilus stored in 100 mg/mL trehalose and 1 mg/mL PVA +50 mg/mL trehalose combo showed significantly enhanced survival when compared with those in traditional cryoprotectant (20% [v/v] glycerol), which achieved the survival percentage of only 41.03 ± 0.09%. The effects of the freezing temperature and crystallinity on the survival of S. thermophilus were elucidated.
冷冻保存被广泛用于细菌的长期储存。甘油是传统的冷冻保护剂之一，广泛用于防止细菌冷冻保存过程中的冷冻损伤，尽管它可能对细胞有毒。为了克服这些问题，合成抗冻聚合物也被用作冷冻保护剂来抑制冰的形成。在研究中，我们比较了各种合成抗冻聚合物，包括聚乙烯醇（PVA）、聚乙烯吡咯烷酮、聚乙二醇和葡聚糖与甘油的性能，其中 PVA 在降低冰生长速率方面表现最好。还探讨了甘油、海藻糖与 PVA 对嗜热链球菌存活的影响。值得注意的是，与传统冷冻保护剂（20%[v/v]甘油）相比，储存在 100mg/mL 海藻糖和 1mg/mL PVA+50mg/mL 海藻糖组合中的嗜热链球菌的存活率显著提高，仅为 41.03±0.09%。还阐明了冷冻温度和结晶度对嗜热链球菌存活的影响。
