McIntyre T D, Alpern H P
Pharmacol Biochem Behav. 1986 Nov;25(5):1077-81. doi: 10.1016/0091-3057(86)90088-2.
Evidence supports the notion that differences between the Long-Sleep and Short-Sleep selectively-bred lines of mice are attributable to differences in brain excitability and that these differences are mediated by activity of the GABAergic system. The general applicability of this hypothesis to other populations of mice was tested by using an outbred strain of mice. Specifically, a heterogeneous strain of mice was administered several doses of the hypnotic chlordiazepoxide. Additionally, the indirect GABA agonist AOAA, and the GABA antagonists bicuculline, picrotoxin and pentylenetetrazol were administered to independent groups in conjunction with chlordiazepoxide. The results clearly demonstrate that chlordiazepoxide dose-dependently increased hypnosis, while AOAA enhanced, and the antagonists attenuated sleep time. These findings can be used to support the contention that GABA mediates the bidirectional response of Long-Sleep and Short-Sleep mice to CNS hypnotic-depressants; and, further, show that GABA mediation of sleep time in mice is a general phenomenon.
有证据支持这样一种观点，即长睡眠和短睡眠选择性培育品系小鼠之间的差异可归因于大脑兴奋性的差异，并且这些差异由GABA能系统的活动介导。通过使用远交系小鼠来测试该假设对其他小鼠群体的普遍适用性。具体而言，给一个异质品系的小鼠施用了几种剂量的催眠药氯氮卓。此外，将间接GABA激动剂AOAA以及GABA拮抗剂荷包牡丹碱、印防己毒素和戊四氮与氯氮卓一起施用于独立的组。结果清楚地表明，氯氮卓剂量依赖性地增加催眠作用，而AOAA增强，拮抗剂则缩短睡眠时间。这些发现可用于支持GABA介导长睡眠和短睡眠小鼠对中枢神经系统催眠药双向反应的论点；并且，进一步表明GABA对小鼠睡眠时间的调节是一种普遍现象。
