Key Laboratory of Recycling and Eco-treatment of Waste Biomass of Zhejiang Province, School of Environment and Natural Resources, Zhejiang University of Science and Technology, Hangzhou 310023, China.
Key Laboratory of Recycling and Eco-treatment of Waste Biomass of Zhejiang Province, School of Environment and Natural Resources, Zhejiang University of Science and Technology, Hangzhou 310023, China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 8):127519. doi: 10.1016/j.ijbiomac.2023.127519. Epub 2023 Oct 20.
The solubility of cyclodextrin metal-organic frameworks (CD-MOFs) in aqueous media making it not suitable as sustained-release drug carrier. Here, curcumin-loaded CD-MOFs (CD-MOFs-Cur) was embedded in chitosan-graft-poly(lactic acid) (CS-LA) via a solid-in-oil-in-oil (s/o/o) emulsifying solvent evaporation method forming the sustained-release composite microspheres. At CS-LA concentration of 20 mg/mL, the composite microspheres showed good sphericity. The average particle size of CS-LA/CD-MOFs-Cur (2:1), CS-LA/CD-MOFs-Cur (4:1) and CS-LA/CD-MOFs-Cur (6:1) composite microspheres was about 9.3, 12.3 and 13.5 μm, respectively. The above composite microspheres exhibited various degradation rates and curcumin release rates. Treating in HCl solution (pH 1.2) for 120 min, the average particle size of above microspheres reduced 28.19 %, 24.34 % and 6.19 %, and curcumin released 86.23 %, 78.37 % and 52.57 %, respectively. Treating in PBS (pH 7.4) for 12 h, the average particle size of above microspheres reduced 30.56 %, 26.56 % and 10.66 %, and curcumin released 68.54 %, 54.32 % and 31.25 %, respectively. Moreover, the composite microspheres had a favorable cytocompatibility, with cell viability of higher than 90 %. These composite microspheres open novel opportunity for sustained drug release of CD-MOFs.
环糊精金属有机骨架（CD-MOFs）在水介质中的溶解度使其不适合作为缓控释药物载体。在这里，通过固/油/油（s/o/o）乳化溶剂蒸发法将载姜黄素的 CD-MOFs（CD-MOFs-Cur）嵌入壳聚糖接枝聚（乳酸）（CS-LA）中，形成缓控释复合微球。在 CS-LA 浓度为 20mg/mL 时，复合微球呈现出良好的球形。CS-LA/CD-MOFs-Cur（2:1）、CS-LA/CD-MOFs-Cur（4:1）和 CS-LA/CD-MOFs-Cur（6:1）复合微球的平均粒径约为 9.3、12.3 和 13.5μm。上述复合微球表现出不同的降解率和姜黄素释放率。在 pH 1.2 的 HCl 溶液中处理 120min 后，上述微球的平均粒径分别减少了 28.19%、24.34%和 6.19%，姜黄素的释放量分别为 86.23%、78.37%和 52.57%。在 pH 7.4 的 PBS 中处理 12h 后，上述微球的平均粒径分别减少了 30.56%、26.56%和 10.66%，姜黄素的释放量分别为 68.54%、54.32%和 31.25%。此外，复合微球具有良好的细胞相容性，细胞活力高于 90%。这些复合微球为 CD-MOFs 的缓控释药物释放开辟了新的机会。
