Weng Mingfang, Deng Zhen, Huang Shuijing, Lin Xiaowen, Xu Na, Sun Xinghui, Wu Weizhen, Lu Jun, Wang Dong
Department of Urology, 900TH Hospital of Joint Logistics Support Force, Fujian Medical University, Fuzhou, China.
Department of Neurosurgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
J Biochem Mol Toxicol. 2024 Jan;38(1):e23556. doi: 10.1002/jbt.23556. Epub 2023 Oct 23.
Fraxetin, a natural compound extracted from the Chinese herb Cortex Fraxini, is reported to boast extensive antitumor properties in various cancers. However, whether fraxetin exhibited an anticancer effect on bladder cancer remains unknown. In this study, cell counting kit-8 was utilized to detect cell viability. Flow cytometry analysis was performed for cell apoptosis analysis. Western blot analysis and real-time PCR were used to ascertain gene expression analysis. A mouse bladder cancer xenograft model was established and subjected to fraxetin treatment. Fraxetin reduced the viability of bladder cancer cells, induced apoptosis in vitro, and inhibited the growth of bladder cancer in vivo. Fraxetin inhibited the Akt pathway in J82 cells. In conclusion, the growth inhibitory properties of fraxetin against bladder cancer may be mediated via an Akt inhibitory effect and cell apoptosis promotion.
秦皮素是从中药秦皮中提取的一种天然化合物，据报道在多种癌症中具有广泛的抗肿瘤特性。然而，秦皮素对膀胱癌是否具有抗癌作用仍不清楚。在本研究中，使用细胞计数试剂盒-8检测细胞活力。通过流式细胞术分析进行细胞凋亡分析。采用蛋白质免疫印迹分析和实时聚合酶链反应确定基因表达分析。建立小鼠膀胱癌异种移植模型并给予秦皮素治疗。秦皮素降低了膀胱癌细胞的活力，在体外诱导细胞凋亡，并在体内抑制膀胱癌的生长。秦皮素抑制J82细胞中的Akt信号通路。总之，秦皮素对膀胱癌的生长抑制作用可能是通过抑制Akt信号通路和促进细胞凋亡来介导的。
