Key Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing, China; Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Animal Husbandry and Veterinary College, Jiangsu Vocational College of Agriculture and Forestry, Jurong, Jiangsu, China.
Poult Sci. 2023 Dec;102(12):103159. doi: 10.1016/j.psj.2023.103159. Epub 2023 Oct 2.
The ovarian circadian clock plays a regulatory role in the avian ovulation-oviposition cycle. However, little is known regarding the ovarian circadian clock of geese. In this study, we investigated rhythmic changes in clock genes over a 48-h period and identified potential clock-controlled genes involved in progesterone synthesis in goose ovarian preovulatory granulosa cells. The results showed that BMAL1, CRY1, and CRY2, as well as 4 genes (LHR, STAR, CYP11A1, and HSD3B) involved in progesterone synthesis exhibited rhythmic expression patterns in goose ovarian preovulatory granulosa cells over a 48-h period. Knockdown of BMAL1 decreased the progesterone concentration and downregulated STAR mRNA and protein levels in goose ovarian preovulatory granulosa cells. Overexpression of BMAL1 increased the progesterone concentration and upregulated the STAR mRNA level in goose ovarian preovulatory granulosa cells. Moreover, we demonstrated that the BMAL1/CLOCK complex activated the transcription of goose STAR gene by binding to an E-box motif. These results suggest that the circadian clock is involved in the regulation of progesterone synthesis in goose ovarian preovulatory granulosa cells by orchestrating the transcription of steroidogenesis-related genes.
卵巢生物钟在禽类排卵-产卵周期中发挥调节作用。然而，关于鹅的卵巢生物钟知之甚少。在这项研究中，我们研究了时钟基因在 48 小时内的节律变化，并鉴定了参与鹅卵巢排卵前颗粒细胞中孕酮合成的潜在时钟控制基因。结果表明，BMAL1、CRY1 和 CRY2 以及参与孕酮合成的 4 个基因（LHR、STAR、CYP11A1 和 HSD3B）在鹅卵巢排卵前颗粒细胞中呈 48 小时节律性表达模式。BMAL1 的敲低降低了鹅卵巢排卵前颗粒细胞中的孕酮浓度，并下调了 STAR mRNA 和蛋白水平。BMAL1 的过表达增加了鹅卵巢排卵前颗粒细胞中的孕酮浓度，并上调了 STAR mRNA 水平。此外，我们证明 BMAL1/CLOCK 复合物通过结合 E 盒基序激活鹅 STAR 基因的转录。这些结果表明，生物钟通过协调类固醇生成相关基因的转录，参与调节鹅卵巢排卵前颗粒细胞中孕酮的合成。
