Department of Advanced Materials Engineering, University of Science and Technology, 217 Gajeon-ro, Yeseong-gu, Daejeon, Republic of Korea.
Department of Advanced Biomaterials Research, Ceramic Materials Division, Korea Institute of Materials Science, 797 Changwon-daero, Seongasna-gu, Changwon, Republic of Korea.
Biofabrication. 2023 Nov 7;16(1). doi: 10.1088/1758-5090/ad05e2.
To improve the properties of the hydrogel-based bioinks, a calcium phosphate phase transition was applied, and the products were examined. We successfully enhanced the mechanical properties of the hydrogels by adding small amounts (< 0.5 wt%) of alpha-tricalcium phosphate (-TCP) to photo-crosslinkable gelatin methacrylate (GelMA). As a result of the hydrolyzing calcium phosphate phase transition involving-TCP, which proceeded for 36 h in the cell culture medium, calcium-deficient hydroxyapatite was produced. Approximately 18 times the compressive modulus was achieved for GelMA with 0.5 wt%-TCP (20.96 kPa) compared with pure GelMA (1.18 kPa). Although cell proliferation decreased during the early stages of cultivation, both osteogenic differentiation and mineralization activities increased dramatically when the calcium phosphate phase transition was performed with 0.25 wt%-TCP. The addition of-TCP improved the printability and fidelity of GelMA, as well as the structural stability and compressive modulus (approximately six times higher) after three weeks of culturing. Therefore, we anticipate that the application of calcium phosphate phase transition to hydrogels may have the potential for hard tissue regeneration.
为了改善水凝胶基生物墨水的性能，应用了磷酸钙相转变，并对产物进行了研究。我们通过向可光交联的明胶甲基丙烯酯（GelMA）中添加少量（<0.5wt%）的α-磷酸三钙（-TCP），成功地提高了水凝胶的机械性能。由于涉及-TCP 的磷酸钙相转变在细胞培养基中进行了 36 小时，生成了缺钙羟基磷灰石。与纯 GelMA（1.18kPa）相比，添加 0.5wt%-TCP（20.96kPa）的 GelMA 的压缩模量提高了约 18 倍。尽管在培养的早期阶段细胞增殖减少，但当用 0.25wt%-TCP 进行磷酸钙相转变时，成骨分化和矿化活性显著增加。-TCP 的添加提高了 GelMA 的可打印性和保真度，以及结构稳定性和压缩模量（培养三周后提高约六倍）。因此，我们预计将磷酸钙相转变应用于水凝胶可能具有用于硬组织再生的潜力。
