Sjöquist P O, Kareld L, Wahlund G C, Holmberg S
Scand J Clin Lab Invest. 1986 Nov;46(7):677-83. doi: 10.3109/00365518609083731.
Regional myocardial blood flow was measured with radioactive microspheres before and 1, 3, 6, and 12 h after occlusion of the left anterior descending coronary artery (LAD) in anaesthetized cats. In one group of cats, 1 h after LAD occlusion, moderate normovolaemic haemodilution was started with a dextran 40 solution, which reduced the haematocrit by 20-25% throughout the rest of the experiment. In the other group, the haematocrit was kept at the original level. Infarct size was measured planimetrically 12 h after the coronary ligation, using the tetrazolium staining technique for disclosing the ischaemic myocardium. Haemodilution increased blood flow in the non-ischaemic myocardium and in the so-called 'border zone' comprising both normal, and ischaemic tissue. However, in the severely ischaemic myocardium dependent on collateral blood flow, where there was no admixture of non-ischaemic myocardium, the blood perfusion was not significantly affected by the haemodilution. After 12 h of ischaemia the infarct size in the control group was fully comparable with that in the haemodiluted group: 28 +/- 3 and 27 +/- 4 (percentage of the left ventricle, mean +/- SEM), respectively.
在麻醉猫中，于左冠状动脉前降支（LAD）闭塞前以及闭塞后1、3、6和12小时，用放射性微球测量局部心肌血流量。在一组猫中，LAD闭塞1小时后，开始用右旋糖酐40溶液进行中度等容血液稀释，在实验剩余时间内使血细胞比容降低20 - 25%。在另一组中，血细胞比容保持在原始水平。冠状动脉结扎12小时后，采用四氮唑染色技术显示缺血心肌，通过平面测量法测定梗死面积。血液稀释增加了非缺血心肌以及包含正常和缺血组织的所谓“边缘区”的血流量。然而，在依赖侧支血流的严重缺血心肌中，由于不存在非缺血心肌的混合，血液灌注未受到血液稀释的显著影响。缺血12小时后，对照组的梗死面积与血液稀释组的完全相当：分别为28±3和27±4（左心室的百分比，平均值±标准误）。
