Melchior D L
Science. 1986 Dec 19;234(4783):1577-80. doi: 10.1126/science.3787264.
The application of rapid-freezing techniques to differential scanning calorimetry (DSC) provides a new approach for understanding the organization of lipids in biomembranes. Use of quick-freeze DSC on membranes of mixed lipid composition supports the existence of nonrandom distributions of lipids (domains) in fluid bilayers. In addition to allowing investigations on the organization of lipids in fluid bilayers, the quick-freeze technique now allows calorimetric studies to be carried out on mammalian membranes which, because of their high cholesterol content, have not been previously amenable to study by DSC.
将快速冷冻技术应用于差示扫描量热法（DSC）为理解生物膜中脂质的组织提供了一种新方法。对混合脂质组成的膜使用快速冷冻DSC支持了流体双层中脂质非随机分布（结构域）的存在。除了能够研究流体双层中脂质的组织外，快速冷冻技术现在还允许对哺乳动物膜进行量热研究，由于其高胆固醇含量，此前一直无法用DSC进行研究。
