Carchon H, Serrus M, Eggermont E
Digestion. 1979;19(1):1-5. doi: 10.1159/000198315.
Gliadin, subsequently treated with pepsin, trypsin and pancreatic extract was further digested by small-intestinal mucosal homogenates from 10 control or 8 coeliac children. The amino acids liberated in the incubation mixture were measured and corrected for mucosal damage. In accordance with the data from the literature on adults, the total amount of amino acids released from gliadin peptides by the intestinal mucosa from children with active coeliac disease is significantly lower than that by the mucosa from control subjects. Qualitatively, however, no significant differences for the individual amino acids are observed with the exception of glutamine and proline, so that damaged coeliac mucosa liberates relatively more glutamine but less proline.
麦醇溶蛋白经胃蛋白酶、胰蛋白酶和胰腺提取物处理后，再用10名对照儿童或8名乳糜泻儿童的小肠黏膜匀浆进一步消化。测定孵育混合物中释放的氨基酸，并对黏膜损伤进行校正。根据有关成人的文献数据，患有活动性乳糜泻儿童的肠黏膜从麦醇溶蛋白肽释放的氨基酸总量显著低于对照受试者的肠黏膜释放的量。然而，定性地看，除谷氨酰胺和脯氨酸外，各氨基酸未观察到显著差异，因此受损的乳糜泻黏膜释放相对较多的谷氨酰胺但较少的脯氨酸。
