Guangdong Provincial Key Laboratory of Food, Nutrition and Health, and Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou, 510080, P.R. China.
Department of Statistics and Epidemiology, School of Public Health, Sun Yat-sen University, Guangzhou, 510080, P.R. China.
Mol Nutr Food Res. 2023 Dec;67(24):e2300515. doi: 10.1002/mnfr.202300515. Epub 2023 Oct 24.
Substituting plant protein for animal protein has emerged as a promising strategy for managing atherogenic lipids. However, the impact of long-term intake of a high plant protein diet (HPD) on hepatic lipid disorder remains unclear.
Eight-week-old apolipoprotein E deficient (apoE ) mice are fed with either a normal protein diet (NCD) or HPD for 12 weeks. HPD intervention results in decreased body weight accompanied by increased energy expenditure, with no significant effect on glycemic control. Long-term intake of HPD improves the serum and hepatic lipid and cholesterol accumulation by suppressing hepatic squalene epoxidase (SQLE) expression, a key enzyme in cholesterol biosynthesis. Integrated analysis of 16S rDNA sequencing and metabolomics profiling reveals that HPD intervention increases the abundance of the Lachnospiraece family and serum levels of 12,13-DiHOME. Furthermore, in vivo studies demonstrate that 12,13-DiHOME significantly inhibits lipid accumulation, as well as SQLE expression induced by oleic acid in HepG2 cells.
Diet rich in plant protein diet alleviates hyperlipidemia via increased microbial production of 12,13-DiHOME.
用植物蛋白替代动物蛋白已成为一种有前途的策略，可用于治疗动脉粥样硬化脂质。然而，长期摄入高植物蛋白饮食（HPD）对肝脏脂质紊乱的影响尚不清楚。
将 8 周龄载脂蛋白 E 缺陷（apoE）小鼠分别用正常蛋白饮食（NCD）或 HPD 喂养 12 周。HPD 干预导致体重减轻，同时伴有能量消耗增加，但对血糖控制没有显著影响。长期摄入 HPD 通过抑制胆固醇生物合成的关键酶——鲨烯环氧化酶（SQLE）的表达，改善了血清和肝脏脂质及胆固醇的积累。16S rDNA 测序和代谢组学分析综合结果表明，HPD 干预增加了厚壁菌门的丰度和血清 12,13-DiHOME 水平。此外，体内研究表明，12,13-DiHOME 可显著抑制油酸诱导的 HepG2 细胞中脂质积累和 SQLE 表达。
富含植物蛋白的饮食通过增加微生物产生的 12,13-DiHOME 缓解了高脂血症。
