Zhang Zeye, Zhang Zexin, Song Jinqin, Wu Wenfeng, Chen Yiqi, Li Jing, Wang Yongchen, Zhao Piwen
School of Life Sciences, Beijing University of Chinese Medicine, Beijing, 100029, China.
The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China.
Heliyon. 2023 Oct 12;9(10):e20709. doi: 10.1016/j.heliyon.2023.e20709. eCollection 2023 Oct.
The targets and mechanisms of Si-Wu-Tang (SWT) against (Breast cancer) BRCA were identified and a survival model and nomogram was construted by network pharmacology, bioinformatic analysis and experiments. A total of 72 anti-breast cancer SWT targets were selected, among which eleven genes () were used to construct a novel prognostic model of breast cancer. The anti-breast cancer activity of SWT was related to the modulation of the receptor tyrosine kinases signaling pathways. Moreover, two compounds, mairin and senkyunone were found to bind directly to ITGB3 and RORB proteins. Finally, mRNA and protein expression of ITGB3 and RORB was observed to be significantly down-regulated after incubation of MCF-7 cells with SWT. Overall, our results indicated that mairin and senkyunone were the key ingredients present in SWT, and ITGB3 as well as RORB proteins were the major targets affected by SWT. The prognostic model can be used to predict the outcome of BRCA patients.
通过网络药理学、生物信息学分析和实验，确定了四物汤（SWT）抗乳腺癌（BRCA）的靶点和机制，并构建了生存模型和列线图。共筛选出72个四物汤抗乳腺癌靶点，其中11个基因用于构建新型乳腺癌预后模型。四物汤的抗乳腺癌活性与受体酪氨酸激酶信号通路的调节有关。此外，发现两种化合物，马里宁和川芎嗪能直接与ITGB3和RORB蛋白结合。最后，在用四物汤处理MCF-7细胞后，观察到ITGB3和RORB的mRNA和蛋白表达显著下调。总体而言，我们的结果表明，马里宁和川芎嗪是四物汤中的关键成分，ITGB3和RORB蛋白是受四物汤影响的主要靶点。该预后模型可用于预测BRCA患者的预后。
