Key Laboratory of Basic and New Drug Research in Chinese Medicine, Shaanxi University of Traditional Chinese Medicine, Xianyang, Shaanxi 712046, China.
Key Laboratory of Basic and New Drug Research in Chinese Medicine, Shaanxi University of Traditional Chinese Medicine, Xianyang, Shaanxi 712046, China; Shaanxi Provincial Administration of Traditional Chinese Medicine Key Research Laboratory of Pharmacokinetic Mechanism and Material Basis of Traditional Chinese Medicine, Shaanxi 712046, China.
Biomed Pharmacother. 2023 Dec;168:115727. doi: 10.1016/j.biopha.2023.115727. Epub 2023 Oct 24.
The purpose of this study was to investigate the mechanism through which rosemary essential oil treats atopic dermatitis.
A dinitrochlorobenzene (DNCB)-induced atopic dermatitis mouse model was established and treated with low (1%), medium (2%), and high (4%) doses of Rosmarinus officinalis essential oil (EORO). Serum levels of interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) in each group were determined using enzyme-linked immunosorbent assay (ELISA). Skin tissues were stained with hematoxylin-eosin and toluidine blue. We used network pharmacology and molecular docking techniques to verify the biological activity of essential proteins and their corresponding compounds in the pathway. Gas chromatography-mass spectrometry (GC-MS) was used for metabolomics analysis and multivariate statistical analysis of mouse serum to screen differential metabolites and metabolic pathway analysis. Protein expression of p-JAK1, CD4+ cells, and IL-4 in the skin tissue was detected by immunohistochemistry analysis. Protein levels of STAT3, p-STAT3, P65, and p-P65 in damaged skin tissues were detected using western blotting.
The skin of mice in the model group showed different degrees of erythema, dryness, scratches, epidermal erosion and shedding, and crusting. After treatment, the serum levels of IL-6 and TNF-α in EORO group were significantly decreased, and the expression of p-JAK1,CD4 + cells, IL-4, p-P65 / P65 and p-STAT3 / STAT3 proteins in skin tissues were decreased.
EORO can effectively improve DNCB-induced AD-like skin lesions in mice by regulating the JAK/STAT/NF-κB signaling pathway, thereby reducing the production of downstream arachidonic acid metabolites, inhibiting skin inflammation, and restoring epidermal barrier function.
本研究旨在探讨迷迭香油治疗特应性皮炎的作用机制。
建立二硝基氯苯（DNCB）诱导的特应性皮炎小鼠模型，并用低（1%）、中（2%）、高（4%）剂量迷迭香油（EORO）进行治疗。采用酶联免疫吸附试验（ELISA）检测各组血清中白细胞介素（IL）-6和肿瘤坏死因子-α（TNF-α）的水平。用苏木精-伊红和甲苯胺蓝对皮肤组织进行染色。我们运用网络药理学和分子对接技术验证了该通路中关键蛋白及其对应化合物的生物活性。采用气相色谱-质谱联用（GC-MS）对小鼠血清进行代谢组学分析和多变量统计分析，以筛选差异代谢物和代谢途径分析。采用免疫组化分析检测皮肤组织中 p-JAK1、CD4+细胞和 IL-4 的蛋白表达，采用蛋白质印迹法检测损伤皮肤组织中 STAT3、p-STAT3、P65 和 p-P65 的蛋白水平。
模型组小鼠皮肤出现不同程度的红斑、干燥、抓痕、表皮糜烂和脱落、结痂。经 EORO 治疗后，EORO 组血清中 IL-6 和 TNF-α 水平显著降低，皮肤组织中 p-JAK1、CD4+细胞、IL-4、p-P65/P65 和 p-STAT3/STAT3 蛋白的表达均降低。
EORO 可通过调节 JAK/STAT/NF-κB 信号通路有效改善 DNCB 诱导的 AD 样皮肤损伤，从而减少下游花生四烯酸代谢物的产生，抑制皮肤炎症，恢复表皮屏障功能。
