Department of Chinese Medical and Natural Medicines, School of Pharmacy, Air Force Medical University, Xi'an 710032, China.
Department of Chinese Medical and Natural Medicines, School of Pharmacy, Air Force Medical University, Xi'an 710032, China.
Chin J Nat Med. 2023 Oct;21(10):789-800. doi: 10.1016/S1875-5364(23)60486-8.
Five new saponins, including three steroid saponins, paristenoids A-C (1-3), and two triterpenoid saponins, paristenoids D-E (4-5), along with four known ones (6-9) were isolated from the rhizomes of Paris polyphylla var. stenophylla. The structures of the isolated compounds were identified mainly by detailed spectroscopic analysis, including extensive 1D and 2D NMR, MS, as well as chemical methods. Compound 3 is a new cyclocholestanol-type steroidal saponin with a rare 6/6/6/5/5 fused-rings cholestanol skeleton, and this skeleton has been first found from the genus Paris. The cytotoxicities of the isolated compounds against three human three glioma cell lines (U87MG, U251MG and SHG44) were evaluated, and compound 7 displayed certain inhibitory effect with IC values of 15.22 ± 1.73, 18.87 ± 1.81 and 17.64 ± 1.69 μmol·L, respectively.
从云南重楼的根茎中分离得到了五个新的甾体皂甙，包括三个甾体皂甙，Paristenoids A-C（1-3），和两个三萜皂甙，Paristenoids D-E（4-5），以及四个已知的化合物（6-9）。通过详细的光谱分析，包括广泛的 1D 和 2D NMR、MS 以及化学方法，确定了分离得到的化合物的结构。化合物 3 是一种新型的环羊毛甾烷型甾体皂甙，具有罕见的 6/6/6/5/5 稠合环羊毛甾烷骨架，这种骨架首次从巴黎属中发现。对分离得到的化合物对三种人神经胶质瘤细胞系（U87MG、U251MG 和 SHG44）的细胞毒性进行了评价，化合物 7 表现出一定的抑制作用，IC 值分别为 15.22±1.73、18.87±1.81 和 17.64±1.69μmol·L。
