Department for Cardiac Surgery, Medical Faculty, Otto von Guericke University, Magdeburg, Germany.
Laboratory of Fluid Dynamics and Technical Flows, Otto von Guericke University, Magdeburg, Germany.
Sci Rep. 2023 Oct 25;13(1):18274. doi: 10.1038/s41598-023-45451-2.
Revascularization via coronary artery bypass grafting (CABG) to treat cardiovascular disease is established as one of the most important lifesaving surgical techniques worldwide. But the shortage in functionally self-adaptive autologous arteries leads to circumstances where the clinical reality must deal with fighting pathologies coming from the mismatching biophysical functionality of more available venous grafts. Synthetic biomaterial-based CABG grafts did not make it to the market yet, what is mostly due to technical hurdles in matching biophysical properties to the complex demands of the CABG niche. But bacterial Nanocellulose (BNC) Hydrogels derived by growing biofilms hold a naturally integrative character in function-giving properties by its freedom in designing form and intrinsic fiber architecture. In this study we use this integral to combine impacts on the luminal fiber matrix, biomechanical properties and the reciprocal stimulation of microtopography and induced flow patterns, to investigate biomimetic and artificial designs on their bio-functional effects. Therefore, we produced tubular BNC-hydrogels at distinctive designs, characterized the structural and biomechanical properties and subjected them to in vitro endothelial colonization in bioreactor assisted perfusion cultivation. Results showed clearly improved functional properties and gave an indication of successfully realized stimulation by artery-typical helical flow patterns.
通过冠状动脉旁路移植术（CABG）进行血管重建以治疗心血管疾病已被确立为全球最重要的救生手术技术之一。但功能自适应自体动脉的短缺导致临床实际情况必须应对来自更可用静脉移植物的不匹配生物物理功能的病变。基于合成生物材料的 CABG 移植物尚未进入市场，这主要是由于在将生物物理特性匹配到 CABG 生态位的复杂要求方面存在技术障碍。但是，通过生长生物膜而衍生的细菌纳米纤维素（BNC）水凝胶通过其在设计形式和内在纤维结构方面的自由度，具有赋予功能的天然整合特性。在这项研究中，我们利用这种整体特性，将对管腔纤维基质、生物力学特性以及微地形和诱导流型的相互刺激的影响结合起来，研究仿生和人工设计对其生物功能的影响。因此，我们以独特的设计生产管状 BNC 水凝胶，对其结构和生物力学特性进行了表征，并在生物反应器辅助灌注培养中对其进行了体外内皮定植。结果清楚地表明功能特性得到了改善，并表明成功地实现了动脉典型螺旋流模式的刺激。
