Department of Dentistry, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica Str. 69, Kragujevac, Republic of Serbia.
Department of Science, Institute for Information Technologies, University of Kragujevac, Kragujevac, Serbia.
Mol Cell Biochem. 2024 Oct;479(10):2679-2695. doi: 10.1007/s11010-023-04868-z. Epub 2023 Oct 25.
This study aimed to evaluate the dental pulp responses to recombinant human erythropoietin (rhEPO) and/or mineral trioxide aggregate (MTA) in pulp capping of inflamed dental pulp in vivo.
In accordance with ARRIVE guidelines, pulp inflammation was induced by exposing the maxillary first molars (n = 64) of Wistar rats (n = 32) to the oral environment for two days. The exposed pulps were randomly assigned four groups based on the pulp capping material: rhEPO, MTA, MTA + rhEPO, or an inert membrane. An additional eight rats formed the healthy control group. After four weeks, the animals were euthanized, and histological, qRT-PCR, and spectrophotometric techniques were employed to analyze the left maxillary segments, right first maxillary molars, and blood samples, respectively. Statistical significance was set at p < 0.05 and < 0.001.
Pulp capping with rhEPO, MTA, or MTA + rhEPO resulted in lower inflammation and higher mineralization scores compared to untreated control. MTA + rhEPO group exhibited significantly decreased expression of tumor necrosis factor-alpha, and interleukin 1-beta, while MTA group showed substantially reduced expression of interferon-gamma. Both rhEPO and MTA + rhEPO groups presented elevated dentin matrix protein 1 levels compared to untreated control. Furthermore, pulp capping with rhEPO and/or MTA led to increased transforming growth factor-beta 1 expression and reductions of pro-inflammatory/immunoregulatory cytokine ratios and prooxidative markers. Pulp capping with rhEPO also resulted in increase of systemic antioxidative stress markers.
Capping with rhEPO or MTA + rhEPO resulted in a favorable effect that was similar or even superior to that of MTA.
本研究旨在评估重组人红细胞生成素（rhEPO）和/或三氧化矿物凝聚体（MTA）在体内发炎牙髓盖髓中的牙髓反应。
根据 ARRIVE 指南，通过将 Wistar 大鼠（n=32）的上颌第一磨牙（n=64）暴露于口腔环境中两天来诱导牙髓炎症。根据牙髓盖髓材料将暴露的牙髓随机分为四组：rhEPO、MTA、MTA+rhEPO 或惰性膜。另外 8 只大鼠组成健康对照组。四周后，处死动物，分别采用组织学、qRT-PCR 和分光光度技术分析左侧上颌段、右侧第一上颌磨牙和血液样本。统计学意义设定为 p<0.05 和<0.001。
与未治疗对照组相比，rhEPO、MTA 或 MTA+rhEPO 盖髓可降低炎症程度，提高矿化程度。rhEPO、MTA+rhEPO 组肿瘤坏死因子-α和白细胞介素 1-β的表达显著降低，而 MTA 组干扰素-γ的表达显著降低。rhEPO 和 MTA+rhEPO 组的牙本质基质蛋白 1 水平均高于未治疗对照组。此外，rhEPO 和 MTA+rhEPO 盖髓可增加转化生长因子-β 1 的表达，降低促炎/免疫调节细胞因子比值和促氧化标志物。rhEPO 盖髓还导致全身抗氧化应激标志物增加。
rhEPO 或 MTA+rhEPO 盖髓的效果与 MTA 相似甚至优于 MTA。
