Department of Pathogen Biology, School of Basic Medical Sciences, Tianjin Medical University, No. 22 Qi-Xiang-Tai Road, Tianjin, 300070, China.
Arch Virol. 2023 Oct 27;168(11):282. doi: 10.1007/s00705-023-05899-z.
miR-HCC2 has been reported to markedly promote the growth, metastasis, and stemness of hepatocellular carcinoma (HCC) cells in vitro and in vivo. Deep sequencing showed that miR-HCC2 was significantly upregulated in hepatitis B virus (HBV)-positive (HBV) HCC tissue samples compared with HBV-negative (HBV) HCC tissue samples. miR-HCC2 expression was further evaluated in HCC tissues and cells, and the expression of miR-HCC2 was found to be significantly higher in HBV HCC tissues and cells than in HBV HCC tissues and cells, suggesting that high miR-HCC2 expression could be induced by HBV infection. To explore the relationship between miR-HCC2 and HBV, we investigated the effect of miR-HCC2 on HBV antigen expression, transcription, and replication. We found that miR-HCC2 was involved in the negative feedback regulation of HBV replication. Further mechanistic studies revealed that miR-HCC2 suppressed HBV replication by inhibiting the activity of the enhancer I/X promoter. Our study demonstrates the effect of the inhibition of miR-HCC2 on HBV gene expression and replication, which can help to illustrate the complex regulatory network involving host miRNAs and HBV.
miR-HCC2 已被报道可显著促进肝癌（HCC）细胞的体外和体内生长、转移和干细胞特性。深度测序显示，miR-HCC2 在乙型肝炎病毒（HBV）阳性（HBV）HCC 组织样本中明显上调，而在 HBV 阴性（HBV）HCC 组织样本中下调。miR-HCC2 的表达在 HCC 组织和细胞中进一步进行了评估，发现 miR-HCC2 在 HBV HCC 组织和细胞中的表达明显高于 HBV HCC 组织和细胞，表明高 miR-HCC2 表达可能是由 HBV 感染诱导的。为了探讨 miR-HCC2 与 HBV 之间的关系，我们研究了 miR-HCC2 对 HBV 抗原表达、转录和复制的影响。我们发现 miR-HCC2 参与了 HBV 复制的负反馈调节。进一步的机制研究表明，miR-HCC2 通过抑制增强子 I/X 启动子的活性抑制 HBV 复制。我们的研究证明了抑制 miR-HCC2 对 HBV 基因表达和复制的影响，这有助于阐明涉及宿主 miRNAs 和 HBV 的复杂调控网络。
