School of Basic Medicine, Guizhou Medical University, Guiyang, China.
Key Laboratory of Agricultural and Animal Products Storage and Processing Technology, Guizhou University, Guiyang, China.
J Med Food. 2023 Nov;26(11):831-842. doi: 10.1089/jmf.2023.K.0118. Epub 2023 Oct 27.
To explore hypoglycemic effect of wild tratt (RRT) juice at different altitudes on type 1 diabetes mellitus (T1DM). The T1DM mouse model was induced by streptozotocin (STZ), and the experiment included a normal group (NC), model group (MC), wild RRT juice groups high (HF), medium (MF), low altitude (DF) and cultivated control group (PC). During experiment, food intake, water intake, body weight, and fasting blood glucose were measured. After 28 days of administration, glucose tolerance, glycogen level, lipid profiles, and antioxidation levels in serum and liver were measured, and histomorphological changes of liver and kidney were observed by hematoxylin and eosin staining. The results showed that wild RRT juice reduced blood glucose level, alleviated liver and kidney tissue damage, improved glucose and lipid metabolism disorders and attenuated oxidative damage in T1DM mice. Western blot showed that wild RRT juice at grown at different altitudes significantly increased protein abundance of PI3K, Akt, and GLUT2 in liver of T1DM mice. In conclusion, wild RRT juice from different altitudes improved glucose and lipid metabolism disorders and oxidative damage in T1DM mice, which may be attributed to activation of PI3K/Akt pathway. Overall effect: MF > PC > HF > DF.
探讨不同海拔野生菝葜（RRT）汁对 1 型糖尿病（T1DM）的降血糖作用。采用链脲佐菌素（STZ）诱导 T1DM 小鼠模型，实验分为正常组（NC）、模型组（MC）、野生 RRT 高海拔（HF）、中海拔（MF）、低海拔（DF）汁组和栽培对照组（PC）。实验过程中测量了摄食量、饮水量、体重和空腹血糖。给药 28 天后，测量了血清和肝脏中的葡萄糖耐量、糖原水平、血脂谱和抗氧化水平，并通过苏木精和伊红染色观察了肝和肾的组织形态变化。结果表明，野生 RRT 汁降低了 T1DM 小鼠的血糖水平，减轻了肝、肾组织损伤，改善了糖、脂代谢紊乱，减轻了氧化损伤。Western blot 显示，不同海拔生长的野生 RRT 汁显著增加了 T1DM 小鼠肝脏中 PI3K、Akt 和 GLUT2 蛋白的丰度。综上所述，不同海拔的野生菝葜汁改善了 T1DM 小鼠的糖、脂代谢紊乱和氧化损伤，这可能归因于 PI3K/Akt 通路的激活。整体效果：MF＞PC＞HF＞DF。
